Browsing by Author "Teruna, Hilwan Yuda"

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    AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL 30% KULIT BUAH MANGGIS (Garcinia mangostana L.) SEBELUM DAN SESUDAH PROSES PENGERINGAN DALAM OVEN 60OC
    (2016-05-10) Simbolon, Lidya Purnama; Nugroho, Titania Tjandrawati; Teruna, Hilwan Yuda
    Ethanol extracts of mangosteen fruit rind contain high antioxidant activity that is heat labile. Drying of ethanol extracts containing antioxidant activity ideally is carried out at low temperatures using a vacuum freeze dryer. In the absence of a vacuum freeze dryer other methods must be used, such as oven drying but at temperatures with minimal loss of the antioxidant activity. To evaluate the effects of the drying process, the antioxidant activities of mangosteen fruit rind 30% ethanol extracts before and after oven drying at 60oC was compared to each other. Dried powdered mangosteen fruit rind was incubated in 30% ethanol for 8 days at 40ºC and 100 rpm in a shaking incubator. After incubation, the mixture was filtered to separate the ethanol extract from the remaining solids. The ethanol solvent from the filtered extract was evaporated by rotary vacuum evaporation at 50oC, and the semi-dried residue was further oven dried at 60oC to constant weight. The antioxidant activity of the semi-dried extract residue and oven dried extract was analyzed using the 2,2 diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that the IC50 value of the antioxidant activity from mangosteen fruit rind 30% ethanol extract before the drying process was (42.78±1.54) μg/mL, and after the drying process was (21.30±2.29) μg/mL. Unpaired student t analysis to compare the average IC50 values of the 30% extracts before and after drying showed that there was a significant increase (p<0,05) in the antioxidant activity after the 60oC oven drying process.
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    AKTIVITAS TOKSISITAS DAN ANTIBAKTERI METABOLIT SEKUNDER EKSTRAK ETIL ASETAT DAGING BIJI TUMBUHAN KLUWAK (Pangium edule Reinw)
    (2016-05-10) Ismar, Rani; Teruna, Hilwan Yuda; Jasril
    Kluwak (Pangium edule Reinw) is a plant that has a lot of potential but has not been widely studied. Kluwak seeds consist 33.16% methyl linoleate and 44.93% methyl oleate that can be used as source of essential oil. The extraction of kluwak oil was carried out by maceration using ethyl acetate because the extraction’s temperature was below the solvent’s boiling point so that the degradation of oil component as the effect of heat can be avoided. Our finding showed that 12.03% kluwak oil consist 4.68% patchouli alcohol; 12.26% methyl palmitate; 33.16% methyl linoleate; 44.93% methyl oleate and 4.97% methyl stearate. Toxicity activity assay using BSLT method showed that kluwak oil was not toxic againts Artemia salina with LC50 values more than 1000 ppm. The antibacterial activity determined by agar diffusion method in concentration of 30; 60; 90 and 150 𝜇𝜇g/disk. Bacteria that have been used for this evaluation were Escherichia coli and Bacillus subtilis. This antibacterial assay indicated that the kluwak oil has activity against both bacterial tested.
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    Analisis Molecular Docking Diterpen Kuinon terhadap Reseptor Covid-19
    (2020-08) Khatami, Fajri; Teruna, Hilwan Yuda
    Coronavirus disease or Covid-19 is a disease caused by a new type of corona virus, SARS-CoV-2. This disease first appeared in Wuhan, China and has now become a pandemic in the world. An enzyme that was important in mediating replication and transcription of SARS-CoV-2 was the main protease (Mpro). This study aims to discover compounds that could inhibit the main Mpro of covid-19, through the study of molecular docking of diterpenoid abieten derivatives on PDB ID : 6LU7. The molecular docking study was carried out using Autodock4 software and visualized using PyMOL and Discovery studio. The results of the method validation or redocking showed a Root Mean Square Deviation (RMSD) valued was 1.85 Å. The molecular docking study of 3 derivatives of diterpenoid abieten (6-acetyl7-hydroxyroileanone, 7-hydroxyroileanone and another abieten (CH-6)) showed the binding energy values were -9.07; -8.22 and -7.94 kcal/mol. These results indicate the 6-acetyl7-hydroxyroileanone and 7-hydroxyroileanone compounds have a stronger affinity for the main protease enzyme (Mpro) compared to the original ligand which have binding energy of -7.39 kcal/mol. In addition, the type of hydrogen bond in 6-acetyl7-hydroxyroileanone has 6 bonds which were same as the hydrophilic bond in the native ligand. This research predicted that the 6-acetyl7-hydroxyroileanone compound can be used as an inhibitor of the Covid-19 main protease enzyme (Mpro).
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    ISOLASI DAN IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DARI EKSTRAK ETIL ASETAT PADA KULIT BATANG MEDANG LENDIR (Litsea glutinosa)
    (2020-08) Putri, Sherinda Amelia; Teruna, Hilwan Yuda
    The stem bark of Litsea glutinosa (Medang lendir) from the Lauraceae family has been used as mosquito repellent by indigeneous community in Kampar Kiri Hulu sub-district, Riau Province. In this study, a secondary metabolite was isolated from ethyl acetate extract of Litsea glutinosa stem bark. The pure compound from vial of 6th was separated by flash chromatography method. The UV/VIS spectrum showed maximum absorption at 265 nm. The characterization of the compound was determined by FT-IR spectroscopy which showed the presence of the OH group, cyclic alkene C=C, aromatic carbonyl ester C=O, aliphatic C-H, and ester C-O.
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    ISOLASI DAN KARAKTERISASI METABOLIT SEKUNDER DARI EKSTRAK DIKLOROMETANA TANAMAN MIANA MERAH (Coleus hybridus)
    (2020-08) Andini, Nurul Rizki; Teruna, Hilwan Yuda
    The Coleus hybridus plant, also known as red miana, is a traditional medicinal plant of the genus Coleus from the family Lamiaceae. This plant posses various bioactivities such as antioxidants and antibacterial, antivirus and anti-inflamation. The purpose of this research was to isolate secondary metabolites from dichloromethane extract of the red miana plant. Isolation was done by Chromatotron® in fraction 3 resulting from VLC. The isolated compound was coded as CH-D-01. The UV-Vis spectrum showed maximum absorption at wavelengths of 365,5 and 218 nm (C=C). The FT-IR spectra showed vibration at wavenumber of (cm-1) 3479 (OH); 3089 (=C-H alifatik); 2905, 2836, 2767 (C-H siklik); 1680 (C=O); 1605 (C=C); dan 1280, 1245, 1190 (C-O).
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    ISOLASI DAN KUANTIFIKASI RNA TOTAL PADA SAMPEL LIVER YANG DIINDUKSI D-GALN/LPS DENGAN METODE SPEKTROFOTOMETRI
    (Elfitra, 2023-06) Fitriani, Salsabila; Teruna, Hilwan Yuda
    Liver disease is a serious global health issue. One of the causes of liver disease is hepatitis, which is the seventh leading cause of death worldwide. Research on drugs for liver damage cases has been extensively conducted. The effectiveness and toxicity of a compound on liver cells can be tested by analyzing gene expression, which starts with the isolation and quantification of RNA. The isolation process is carried out to obtain RNA, which carries genetic information, while quantification is performed to determine the concentration and purity of the isolated RNA. In the research conducted, the isolation was performed using the trizol method, and quantification was done using the spectrophotometry method. Based on the research findings, a significant amount of RNA concentration and good RNA purity were obtained, enabling its use for further analysis.
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    ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI TUMBUHAN Spathoglottis aurea Lindl
    (2016-05-19) Arjinal; Yuharmen; Teruna, Hilwan Yuda
    Isolation of active coumpound of root Spatoglottis aurea Lindl was done with maceration method using methanol. The methanol extract was partitioned with hexane and obtained a total of methanol extract and hexane. Extract methanol and hexane were fractionated with column chromatography and were obtained separation results 4 fractions that is Fm1-Fm4 and Fh1-Fh4 and the fractions 2 (Fh2) of hexane extract was obtained white crystals. From UV spectra of crystal Fh2 showed there was a transition π →π* indicating that there was Fh2 crystal C=C double bond. Fh2 IR spectrum of the compound showed OH group, GC-MS analysis showed the compound has the formula of C29H50O with a peak molecular ion m/z 414 which was a coumpound of β-sitosterol with a melting poin of 131-133 0C. Antibacterial activity test extract of methanol and hexane were not active against bacteria test, while the fraction of Fh1 and Fh2 hexane extract active against bacteria S. aureus dan bakteri E. coli.
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    ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL TUMBUHAN KITOLOD (Isotoma longiflora (Wild.) Presl) TERHADAP Bacillus subtilis DAN Pseudomonas aeruginosa
    (2016-05-10) Paramita, Shinta; Eryanti, Yum; Teruna, Hilwan Yuda
    Isolation and antibacterial assay of Isotoma longiflora (Wild.) Presl plants have been done. The isolation of secondary metabolites of I. longiflora (Wild.) Presl was carried out by maceration method using n-hexane and methanol as solvent. A total of 500 g I. longiflora (Wild.) Presl plants yielded 23.31 g and 55.43 g of n-hexane and methanol extract, respectively. The methanol extract was fractionated using VLC and column chromatography. Antibacterial assay was conducted by the agar diffusion method against Bacillus subtilis and Pseudomonas aeruginosa. The methanol extract and fraction exhibited weak activity against two bacterial tested.
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    ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA METABOLIT SEKUNDER DARI F3 (V3-6) EKSTRAK n-HEKSANA BATANG SEMBUKAN (Paederia scandens)
    (2016-10-19) Dewi, Mirna Wati; Teruna, Hilwan Yuda; Yuharmen
    Sembukan or kasembukan often called kentut-kentut leaves (Paederia scandens) belonging to family Rubiaceae is one of medicinal plant species in Indonesia. Phytochemical test showed that the stem of this plant contains secondary metabolites terpenoids and saponins. Metabolites of the stems were extracted using n-hexane by maceration method. The n-hexane extract obtained was separated using VLC and purified further using flash chromatography. Vials 3 to 6 were combined (called VG1) based on the TLC patterns then recrystallized and resulting orange solids that are not pure. Characterization of VG1 by UV-Vis spectroscopy and FT-IR allegedly showed functional groups C=O and S-H. Antibacterial activity test using agar diffusion method was performed on VG1 and n-hexane extract. Based on the activity test, VG1 compounds have no antibacterial activity against (negative), while the n-hexane extract had a weak activity against B. subtilis and P. aureginosa.
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    ISOLASI DAN UJI AKTIVITAS ANTIJAMUR SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL KULIT BATANG Goniothalamus sp. (ANNONACEAE)
    (2016-05-02) Adelia, Ezra Tio; Teruna, Hilwan Yuda; Yuharmen
    The aim of this study was to isolate the secondary metabolites of the stem bark of Gonoiothalamus sp. and to determine antifungal activity of methanol extract. The methanol extract was fractionated using column chromatography. One of the fractions was recrystallized and found as was yellowish crystals (F4). Its HPLC chromatogram compared with HPLC chromatogram of pinocembrin. The result showed that he yellowish crystals (F4) was pinocembrin. Antifungal assay was conducted by the agar diffusion method against Candida albicans and Aspergillus niger. The methanol extract and F4 exhibited weak activity against Candida albicans with inhibition zone 9.03 mm for methanol extract and 8.50 mm for F4, while methanol extract and F4 was inactive againts Aspergillus niger.
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    ISOLASI DAN UJI AKTIVITAS ANTIMIKROBA SENYAWA METABOLIT SEKUNDER DARI EKSTRAK KULIT MELON (Cucumis melo L)
    (2016-10-19) Nurhidayah; Yuharmen; Teruna, Hilwan Yuda
    This study aims to determine the presence of secondary metabolites contained in the melon peel and their antimicrobial activity. Sample was extracted by maceration method using hot aquadest (60℃). The gravity column chromatography of the melon peel extract gave 5 combined fractions. These fractions were subjected to antimicrobial test and FTIR was recorded. Antimicrobial activity test using agar diffusion method with a concentration of 600, 800 and 1000 μg/disc for the extract and 1000 μg/disc for the fractions. Identification showed the presence of terpenoid and phenolic compounds. From 1 kg of melon peel gave 61.85 g of black gummy extract and produced 5 yellow liquid fractions. Melon peel extract showed weak activity againts B. subtilis and P. aeruginosa, but not active against A. niger.
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    ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL KULIT BATANG Goniothalamus sp. (ANNONACEAE)
    (2016-05-16) Ani, Tri Murni; Teruna, Hilwan Yuda; Yuharmen
    Isolation of methanol extract of the stem bark Goniothalamus sp. (Annonaceae) have been conducted and DPPH method using microplate reader has been performed for antioxidant activity assay. The methanol extract was fractionated by vacuum liquid chromatography (VLC) and produced nine fractions called FG1 to FG9. FG4 was recrystallized and generated a pure compound FG4, light yellow crystals. HPLC analysis showed FG4 compound is pinocembrin. FG6 was fractionated by using Chromatotron to give five fractions called FG6A to FG6E. Analysis of the FG6C fraction showed FG6C was not pure indicated by multiple peak in HPLC chromatograms. FT-IR spectrum showed OH, C=O, C-O, CH aromatic and CH aliphatic group. In addition, the FG6C gave a distinctive odour. The results showed that the methanol extract, FG6, FG8 and FG6C have antioxidant activity with IC50 value of 331; 201; 259; and 341.68 μg/mL for each sample, while FG4 fraction and pure compound FG4 was inactive as an antioxidant (IC50 >1000 μg/mL).
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    ISOLASI DAN UJI AKTIVITAS PENGHAMBATAN α-GLUKOSIDASE SENYAWA METABOLIT SEKUNDER EKSTRAK n-HEKSANA DARI DAUN NONA MAKAN SIRIH (Clerodendrum thomsoniae Balf.f.)
    (Elfitra, 2022-07) Abelsia, Anggun Ratu; Teruna, Hilwan Yuda
    Clerodendrum thomsoniae is a species of the Lamiaceae family which is used as a medicinal plant. Previous studies on several species of the genus Clerodendrum has shown that this genus has secondary metabolites that have the potential to be antidiabetic, antioxidant, anti-inflammatory, anticancer, and antiasma agents. This study aims to isolate secondary metabolite compounds from the leaves of C. thomsoniae and test the inhibitory activity of the enzyme α-glucosidase. Isolation of the plants leaves has been carried out by the maceration method using methanol solvent and partioned with n-hexane. The metabolites of n-hexane fraction have been separated using the vacuum liquid chromatography method. Fraction 4 of VLC has been re-separated by the flash chromatography method and obtained 93 vials. Purification of the compound was carried out on vials 21-22 and white crystalline solids (10.75 mg) were obtained. Characterization of the compound was carried out by FTIR spectroscopy which showed the presence of OH, aliphatic C-H, CH2, CH3, C=C alkenes cyclic and C-O (alcohol) groups. The melting point of the compound in the range of 140-142°C. The TLC profile of CT-H-V4-FL21 was not similar to β-sitosterol, therefore these two compounds were different. The enzyme inhibition test of α-glucosidase showed this compound had a weak inhibitory activity with an inhibition percentage of 6.60%.
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    ISOLASI DAN UJI ANTIOKSIDAN METABOLIT SEKUNDER DARI EKSTRAK ETIL ASETAT TANAMAN MIANA HIJAU-MERAH (Coleus hybridus)
    (Elfitra, 2022-07) Putri, Irda Wahyuni; Teruna, Hilwan Yuda
    Miana (Coleus hybridus) is a species of the Lamiaceae family. This plant contains medicinal compounds that can be used as pain management, anti-inflammatory, antioxidant and antibacterial agents. Phytochemical tests showed that the green-red miana leaves contain flavonoid, terpenoid, steroid, saponin, phenolic and alkaloid. This study aimed to isolate secondary metabolites from green-red miana leaves and to test the antioxidants activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Secondary metabolite compounds from plants were isolated by maceration using methanol as solvent and partitioned using n-hexane and ethyl acetate as solvents. Vacuum liquid chromatography (VLC) and flash column chromatography were used to separated the compounds in the viscous ethyl acetate fraction. Brown solids were obtained in vial 38 as much as 17 mg which was coded as CHHM-EA-V6-FL38 and the thin layer chromatography test still showed 2 spots on the plate. The CHHM-EA-V6-FL38 subfraction was characterized by FT-IR spectroscopy. Based on the IC50 results of the CHHM-EA-V6-FL38 subfraction, it showed an IC50 value of 29.53 μg/mL at a concentration of 100 g/mL which indicated that this subfraction could be concluded to be active in the antioxidant test.
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    ISOLASI DAN UJI ANTIOKSIDAN METABOLIT SEKUNDER EKSTRAK METANOL KITOLOD (Isotoma longiflora (Wild.) Presl)
    (2016-05-16) Rondang; Teruna, Hilwan Yuda; Eryanti, Yum
    Isotoma longiflora (Wild.) Presl.is one species of medicinal plants found in tropical and subtropical regions. Isolation and qualitative antioxidant assay of I. longiflora have been done. The result of maceration yielded 23.31 g and 55.43 g of n-hexane and methanol extract, respectively. The fractionations of methanol extract was conducted by VLC, column chromatography, and column chromatography Sephadex LH-20. Qualitative antioxidant assay of methanol extract and fractions had been done using DPPH (1,1-diphenyl–2–picryl hydrazyl) method. The result of antioxidant assay showed that methanol extract, 3rd, 4th, 5th and 6th (F9-F11) fraction possessed antioxidant activity against DPPH radical. The purity of isolated compound was analyzed by HPLC. The chromatogram of HPLC showed that compound was not pure.
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    ISOLASI DAN UJI ANTIOKSIDAN METABOLIT SEKUNDER EKSTRAK n-HEKSANA BATANG MIANA MERAH HATI (Coleus hybridus)
    (Elfitra, 2022-07) Jannah, Raudatul; Teruna, Hilwan Yuda
    Miana plant (C. hybridus) (Lamiaceae family) which is widely known by the people as an ornamental plant and traditional medicine. This study aims to isolate the chemical compounds contained in maroon miana stem extracted using methanol as a solvent and to analyze the antioxidant activity using the DPPH method. Extraction was carried out with the maceration method using methanol as a solvent. The macerated extract was partitioned with n-hexane and ethyl acetate as solvents. The crude extract of n-hexane was separated using flash chromatography method. The secondary metabolite were isolated from the n-hexane extract of the maroon miana stem were called CHM-H1 (180 mg) and CHM-H2 (60 mg), respectively. CHM-H1 and CHM-H2 are thought to be similar to β-sitosterol and daucosterol compounds, respectively. Phytochemical test to maroon miana stem indicated the presence of secondary metabolites of alkaloids, terpenoids/saponins and phenolic groups. The antioxidant test of the n-hexane extract showed that the n-hexane extract had an IC50 value of 378.35 μg/mL and as ascorbic acid 6.70 μg/mL. Ascorbic acid has an AAI value of 11.93 which is classified as very strong, while the n-hexane extract has an AAI value of 0.21 which is classified as weak.
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    ISOLASI DAN UJI ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER EKSTRAK n-HEKSANA DARI BATANG BENALU KOPI Loranthus ferrugineus
    (perpustakaan UR, 2021-09) Fernando, Anggi; Teruna, Hilwan Yuda
    A coffee tree parasite (Loranthus ferrugineus) is a type of parasitic plant that is widely used as a medicinal plant. This study aimed to isolate and characterize the pure compound obtained from the n-hexane extract of the its stem and also to perform antioxidant tests on the extract. Isolation process was carried out by the maceration method in using n-hexane as solvents, repectively. The plant extracts were dried and then separated using vacuum liquid chromatography (VLC). A solid fraction were collected and then recrystallized to give a pure compound. This compound has a melting point of 76-78oC. This compound is than coded LF-H-03. Pure compound was characterized using FTIR. The peak of FTIR spectroscopy showed absorption at aliphatic wave numbers 2954 cm-1 (C-H), 1736 cm-1 (C=O) and 1472 cm-1 (CH2). Based on the results of the antioxidant n-hexane extract from the stem of the L. ferrugineus plant were active with IC50 values of 138.93 μg/mL
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    ISOLASI DAN UJI PENGHAMBATAN α-GLUKOSIDASE METABOLIT SEKUNDER DARI EKSTRAK n-HEKSANA TUMBUHAN Peperomia pellucida
    (perpustakaan UR, 2021-08) Marasi, Merina Vita; Teruna, Hilwan Yuda
    Peperomia pellucida is one of species belongs to Piperaceae family which is widely distributed in Indonesia. This plant commonly is used as a traditional medicine by various tribes in Indonesia. Several species of Peperomia are reported have antidiabetic, antioxidant, antibacterial, and antiinflammatory. However, the antidiabetic activity from this plant rarely investigated. The extract and the pure compounds were tested for antidiabetic activity by α-glucosidase inhibitor assay. The inhibitory activity was assayed using microplate reader. The results with inhibition persentage values 0,832 % for n-hexane extract, -0,355 % for PP-H-03 and -1,102 % for PP-H-08. Based from the data the extract and isolated compound showing weak antidiabetic activity compared to positive control of acarbose.
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    ISOLASI DAN UJI PENGHAMBATAN α-GLUKOSIDASE METABOLIT SEKUNDER EKSTRAK n-HEKSANA TANAMAN MIANA HIJAU-MERAH (Coleus hybridus)
    (Elfitra, 2022-07) Sari, Ajeng Defa Risqina; Teruna, Hilwan Yuda
    Green-red miana plant (Coleus hybridus) is an ornamental plant that is widely used as a medicinal plant. This plant belongs to genus Coleus and the family Lamiaceae. The goal of this study was to isolate secondary metabolites of green-red miana (Coleus hybridus) and analyze the inhibition activity of α-glucosidase by in vitro method. This plant was extracted using maceration method with methanol and partitioned with n-hexane and ethyl acetate. The separation of n-hexane extract was carried out using vacuum liquid chromatography followed by separation using flash chromatography. Purity of the compound was analyzed using TLC and melting point 138-140oC. The solids were recrystallized to obtain white crystalline solids with weight of 90,36 mg (coded CHHM-H-V4-FL14). Characterization of the solids using FTIR spectroscopy showed the presence of OH groups, aliphatic CH, methyl (CH3) C=C cyclic alkenes and CO. The result of the characterization with the FTIR as well as phytochemical analysis of the CHHM-H-V4-FL14 were assumed to be a steroid secondary metabolites. Analysis of the inhibition of the α-glucosidase enzyme revealed that the CHHM-H-V4- FL14 percentage of inhibition was 8.441% while the percentage of acarbose inhibition (positive control) was 99.492%. It is estimated that the green-red miana (Coleus hybridus) possesses weak inhibition activity against the α-glucosidase enzyme.
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    ISOLASI DAN UJI TOKSISITAS EKSTRAK ETIL ASETAT DAUN Nerium oleander
    (2016-05-10) Fitria, Nelda; Teruna, Hilwan Yuda; Eryanti, Yum
    The isolation of Nerium oleander was carried out by maceration method using n-hexane and ethyl acetate. The ethyl acetate extract of N. oleander was fractionated by Vacuum Liquid Chromatography (VLC). The fractions with vial number 6 to 9 (Fga) from VLC was separated by column chromatography. The fractions with vial number 6 to 23 (Fgb) from column chromatography was separated by column chromatography. The fractions with vial number 66 to 69 (Fgc) from the second column chromatography was characterized using Ultraviolet-Visible (UV-Vis) and Fourier Transform InfraRed (FT-IR) methods. The toxicity of extract and fraction have been determined by using Brine Shrimp Lethality Test (BSLT) methods. The toxicity result showed that the ethyl acetate extract and fractions of VLC (F4, F5, Fga, F10, F11) have LC50 < 1000 ppm. It showed that extracts and fractions were toxic. However, the toxicity result showed that the fractions of VLC (F1, F2, F3) and Fgc have LC50 > 1000 ppm.