ISOLASI DAN UJI PENGHAMBATAN α-GLUKOSIDASE METABOLIT SEKUNDER EKSTRAK n-HEKSANA TANAMAN MIANA HIJAU-MERAH (Coleus hybridus)
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Date
2022-07
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Elfitra
Abstract
Green-red miana plant (Coleus hybridus) is an ornamental plant that is widely used as a
medicinal plant. This plant belongs to genus Coleus and the family Lamiaceae. The goal
of this study was to isolate secondary metabolites of green-red miana (Coleus hybridus)
and analyze the inhibition activity of α-glucosidase by in vitro method. This plant was
extracted using maceration method with methanol and partitioned with n-hexane and
ethyl acetate. The separation of n-hexane extract was carried out using vacuum liquid
chromatography followed by separation using flash chromatography. Purity of the
compound was analyzed using TLC and melting point 138-140oC. The solids were
recrystallized to obtain white crystalline solids with weight of 90,36 mg
(coded CHHM-H-V4-FL14). Characterization of the solids using FTIR spectroscopy
showed the presence of OH groups, aliphatic CH, methyl (CH3) C=C cyclic alkenes and
CO. The result of the characterization with the FTIR as well as phytochemical analysis
of the CHHM-H-V4-FL14 were assumed to be a steroid secondary metabolites.
Analysis of the inhibition of the α-glucosidase enzyme revealed that the CHHM-H-V4-
FL14 percentage of inhibition was 8.441% while the percentage of acarbose inhibition
(positive control) was 99.492%. It is estimated that the green-red miana
(Coleus hybridus) possesses weak inhibition activity against the α-glucosidase enzyme.
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Keywords
α-glucosidase, Coleus, isolation, spectroscopy
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