AKTIVITAS TOKSISITAS DAN ANTIBAKTERI METABOLIT SEKUNDER EKSTRAK ETIL ASETAT DAGING BIJI TUMBUHAN KLUWAK (Pangium edule Reinw)
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Date
2016-05-10
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Abstract
Kluwak (Pangium edule Reinw) is a plant that has a lot of potential but has not been widely studied. Kluwak seeds consist 33.16% methyl linoleate and 44.93% methyl oleate that can be used as source of essential oil. The extraction of kluwak oil was carried out by maceration using ethyl acetate because the extraction’s temperature was below the solvent’s boiling point so that the degradation of oil component as the effect of heat can be avoided. Our finding showed that 12.03% kluwak oil consist 4.68% patchouli alcohol; 12.26% methyl palmitate; 33.16% methyl linoleate; 44.93% methyl oleate and 4.97% methyl stearate. Toxicity activity assay using BSLT method showed that kluwak oil was not toxic againts Artemia salina with LC50 values more than 1000 ppm. The antibacterial activity determined by agar diffusion method in concentration of 30; 60; 90 and 150 𝜇𝜇g/disk. Bacteria that have been used for this evaluation were Escherichia coli and Bacillus subtilis. This antibacterial assay indicated that the kluwak oil has activity against both bacterial tested.
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Toxicity, antibacterial and kluwak (Pangium edule Reinw)