ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL KULIT BATANG Goniothalamus sp. (ANNONACEAE)
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Date
2016-05-16
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Abstract
Isolation of methanol extract of the stem bark Goniothalamus sp. (Annonaceae) have
been conducted and DPPH method using microplate reader has been performed for
antioxidant activity assay. The methanol extract was fractionated by vacuum liquid
chromatography (VLC) and produced nine fractions called FG1 to FG9. FG4 was
recrystallized and generated a pure compound FG4, light yellow crystals. HPLC
analysis showed FG4 compound is pinocembrin. FG6 was fractionated by using
Chromatotron to give five fractions called FG6A to FG6E. Analysis of the FG6C fraction
showed FG6C was not pure indicated by multiple peak in HPLC chromatograms.
FT-IR spectrum showed OH, C=O, C-O, CH aromatic and CH aliphatic group.
In addition, the FG6C gave a distinctive odour. The results showed that the methanol
extract, FG6, FG8 and FG6C have antioxidant activity with IC50 value of 331; 201; 259;
and 341.68 μg/mL for each sample, while FG4 fraction and pure compound FG4 was
inactive as an antioxidant (IC50 >1000 μg/mL).
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Keywords
Goniothalamus sp, methanol extract, antioxidant activity assay