ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL KULIT BATANG Goniothalamus sp. (ANNONACEAE)

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dc.contributor.authorAni, Tri Murni
dc.contributor.authorTeruna, Hilwan Yuda
dc.contributor.authorYuharmen
dc.date.accessioned2016-05-16T03:43:49Z
dc.date.available2016-05-16T03:43:49Z
dc.date.issued2016-05-16
dc.description.abstractIsolation of methanol extract of the stem bark Goniothalamus sp. (Annonaceae) have been conducted and DPPH method using microplate reader has been performed for antioxidant activity assay. The methanol extract was fractionated by vacuum liquid chromatography (VLC) and produced nine fractions called FG1 to FG9. FG4 was recrystallized and generated a pure compound FG4, light yellow crystals. HPLC analysis showed FG4 compound is pinocembrin. FG6 was fractionated by using Chromatotron to give five fractions called FG6A to FG6E. Analysis of the FG6C fraction showed FG6C was not pure indicated by multiple peak in HPLC chromatograms. FT-IR spectrum showed OH, C=O, C-O, CH aromatic and CH aliphatic group. In addition, the FG6C gave a distinctive odour. The results showed that the methanol extract, FG6, FG8 and FG6C have antioxidant activity with IC50 value of 331; 201; 259; and 341.68 μg/mL for each sample, while FG4 fraction and pure compound FG4 was inactive as an antioxidant (IC50 >1000 μg/mL).en_US
dc.description.sponsorshipFakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttp://repository.unri.ac.id/xmlui/handle/123456789/8382
dc.language.isoenen_US
dc.subjectGoniothalamus spen_US
dc.subjectmethanol extracten_US
dc.subjectantioxidant activity assayen_US
dc.titleISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL KULIT BATANG Goniothalamus sp. (ANNONACEAE)en_US
dc.typestudent Paper Post Degreeen_US

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