ISOLASI DAN UJI AKTIVITAS ANTIMIKROBA SENYAWA METABOLIT SEKUNDER DARI EKSTRAK KULIT MELON (Cucumis melo L)
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Date
2016-10-19
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Abstract
This study aims to determine the presence of secondary metabolites contained in the melon peel and their antimicrobial activity. Sample was extracted by maceration method using hot aquadest (60℃). The gravity column chromatography of the melon peel extract gave 5 combined fractions. These fractions were subjected to antimicrobial test and FTIR was recorded. Antimicrobial activity test using agar diffusion method with a concentration of 600, 800 and 1000 μg/disc for the extract and 1000 μg/disc for the fractions. Identification showed the presence of terpenoid and phenolic compounds. From 1 kg of melon peel gave 61.85 g of black gummy extract and produced 5 yellow liquid fractions. Melon peel extract showed weak activity againts B. subtilis and P. aeruginosa, but not active against A. niger.
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Melon peel, antimicrobial, terpenoid, phenolic