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Browsing by Author "Isda, Mayta Novaliza"

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Effectivenes of Vesicular-arbuscular Mycorrhiza and Bokashi on Mycorrhizal Infection and Seedling Growth of Mangosteen (Garcinia Mangostana L.)
(wahyu sari yeni, 2017-09-25) Martina, Atria; Isda, Mayta Novaliza; Sartika, Endang; Hidayat, Asep
Vesicular-Arbuscular mycorrhizal (VAM) fungi were co-inoculated with Bokashi to Garcinia mangostana to study the effectiveness of microsymbiont to the mycorrhizal infection and seddling of the plants. A factorial in Completely Randomized Design was employed in this study, with three VAM dosage (0,10,20 g/ seedling) and three bokashi (0,65 and 85 g/seedling). The result showed that VAM dosage 20 g/seedling without bokashi provided the best result on the growth of plants. The teratment had VAM infection could reach 100% bright root and less root hair. The VAM dosage 20 g/seedling without bokashi was significant to plant height and dry weight.
The Efficacy Of Spraying The Spores Of Metarhizium anisopliae As A Delivery Tehnique To Control Oryctes rhinoceros L. In Efb Compost
(2017-02-10) Silalahi, Abner J; Ginting, Patra A; Efendi, Oslan; Munawarah, Happy Z; Jannah, Wirdatul; Isda, Mayta Novaliza
Biological control of Oructes rhinoceros using entomopathogen is a promising alternative to the commonly used chemical control, as a part of the integrated pest management in oil palm plantations. The beetle breeds in rotting palm, wood stumps and empty fruit bunches in the field. It is not only a serious pest for young oil palms but for mature palms, especially in peat soil plantations. In some cases the entry hole of the pest on the crown can cause secondary fungy attacks, which cause spear-rot disease. Laboratory trials by Central Plantations Services (CPS) to investigate the growth rates and sporulation capacities of Metarhizium anisopliae showed that 12 days after inoculations, M. aniosoliae had grown fully in petridishes. It then sporulated profusely on corn media in 14 days post inoculation with a population of 2.7 x 109 spores/gram. O. rhinoceros beetle larvae werw obtained from the field and grown in non-sterile empty fruit bunch (EFB) composts, produced using aerated bunker composting anisopliae infected 97% of O.rhinoceros larvae within 14 days. Preliminary field trial was conducted ian a peat soil plantation with a heavy infestation of O.Rhinoceros beetless. The result showed that 88% larvae in the compost were infected within 35 days after treatment with M. anisopliae. The treatment was especially effective when larvae were in the 3rd instars stage. Research had shown that M.anisopliae was effective in controlling O.rhinoceros, our study si the first to show that the fungi can also be inoculated in the EFB compost. Despite their values as organic fertilisers, EFB compost are still not widely used, especially in peat soil plantations, mainly because O. rhinoceros beetles tend to breed in EFB compost. Here the study showed that compost can be used both as organic fertilisers and also as agents for pest control.
Identifica'i'ion Of Period Needed Each Phase In Pyrrosia lanceolata (L.) Farwell Fern Root Cells During Mitosis
(2017-02-10) Roslim, Dewi Indriyani; Isda, Mayta Novaliza
The objectives of this research were to identification of period needed each phase in Pyrrosia lanceolata (L.) Farwell fern root cells during mitosis. Pyrrosia lanceolata (L) farwell ferns were obtained from Pekanbaru, Riau. Each phase in mitosis observed by made root cells fresh specimen by squash method. We found that between 3.20 am. Until 3.35 am, overall phase could observe. This result showed that Pyrrosia lanceolata (L._) farwell fern root cells active mitosis at that period or time. Prophase and telophase would be seen at every specimen which obtained from different time. In contrast, it was difficulty get anaphase and metaphase. Therefore, we could not now make some conclusion about period needed each phase in Pyrosia lanceolale (L.) Farwell fern root cells during mitosis.
INDUKSI KALUS DARI EKSPLAN DAUN IN VITRO KELADI TIKUS (Typhonium sp.) DENGAN PERLAKUAN 2,4-D DAN KINETIN
(2016-01-26) Sitinjak, Marlina Agustina; Isda, Mayta Novaliza; Fatonah, Siti
Rodent tuber (Typhonium sp.) belongs to Araceae that has a potential as medicinal plant. The propagation of rodent tuber was conducted using vegetative propagation from bulbs. This procedure takes a long periode to produce a large quantity of plant. Therefore, an in vitro propagation using callus culture is necessary to solve this problem. This research aimed to determine the best concentration of 2,4-D and kinetin to induce callus of in vitro leaf rodent tuber explants. The research was conducted at the Laboratory Terpadu of Biology, Faculty of Math and Natural Sciences UR from August 2014 to January 2015. This research used in vitro leaft explants of rodent tuber with MS medium. The design used is Randomize Group Design with 10 treatment i.e. (A) Without treatment (Control), (B) 0.5 mg / L 2.4-D, (C) 1 mg / L 2.4-D, (D) 1.5 mg / L 2.4-D, (E) 0.5 mg / L 2.4-D + 0.3 mg / L kinetin, (F) 1 mg / L 2.4-D + 0.3 mg / L kinetin, (G) of 1.5 mg / L 2,4-D + 0.3 mg / L kinetin, (H) 0.5 mg / L 2.4-D + 0.5 mg / L kinetin, (I) 1 mg / L 2.4-D + 0.5 mg / L kinetin, (J) 1.5 mg / l 2,4-D + 0.5 mg / L kinetin. The results showed that explants could not produce callus, the explants response just a swelling leaf explants and browning in leaf margin because of an injuries. The treatment that could give up to 100% swelling response was 2.4-D mg / L single (0.5 mg / L and 1 mg / L) and the combination treatment (0.5 mg / L 2.4-D + 0.3 mg /L kinetin, 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1.5 mg / L 2.4-D + 0.5 mg / L kinetin). The treatments that could maintain 66.67% explants growth was the combination 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1 mg /L 2.4-D + 0.5 mg / L kinetin
Induksi Tunas Dan Pembentukan Akar Dari Eksplan Kotiledon Jeruk Siam (Citrus Nobilis Lour.) Asal Kampar Secara In Vitro
(2017-02-10) Isda, Mayta Novaliza; Fatonah, Siti; Lestari, Wahyu
Tissue culture was an alternative way in micropropagation of siam orange (Citrus nobilis Lour.) from Kampar one of the main commodity in Riau province. Shoot induction as an early stage propagation and the rooting stage were very important parts to produce plantlet in vitro technique. The aim of this study was to determine the best BAP and NAA concentration for shoot induction and rooting of siam orange from Kampar. This study using a randomized block design with faktorial treatment. The results of this study show that the best shoot induction was obtained from cotyledon explant grown on MS medium without BAP. The percentage and the average number of shoot were 91,67% and 1,50 respectively. The combination of MS medium with 1 mg/L NAA was the most optimum concentration in rooting induction of siam orange from Kampar, with the percentage of root growth and the highest average number of root 90% and 6.3 roots respectively.
INDUKSI TUNAS DARI EKSPLAN BIJI MANGGIS (Garcinia mangostana L.) DIBELAH EMPAT PADA MEDIA MS (MURASHIGE-SKOOG) DENGAN PENAMBAHAN BAP DAN MADU SECARA IN VITRO
(2016-01-28) Amin, Nur Aisyah; Isda, Mayta Novaliza; Fatonah, Siti
Mangosteen (Garcinia mangostana L.) is a tropical plant that has export value because it has a rich of antioxidant, xanthone, alpha and beta, that can be used for medicine as an anti-cancer agent. One of the alternative method to produce large number and uniform mangosteen seedlings inshort period can be done using in vitro culture technique. The purpose of this research was to determine the optimal concentration of BAP and honey in inducing shoot from of mangosteen explants from seed that had been divided into four parts and also to determine the best combination to induce mangosteen shoot uisngin vitromethod. The research was conducted at Integrated Biology Laboratory Department of Biologi, Faculty of Mathematics and Natural Sciences, Riau University from January until April 2015. This research used a Completely Randomized Design (CRD) with the concentration of BAP (3; 7 mg/l) and honey (3; 6; 9 ml/l), or in combination on MS medium with 5 replications.The result showed that the treatment on medium supplemented with each BAP and honeyor in combination gave the highest percentage of explant growthand shoot formation (100%)while the medium supplement with 7 mg/l BAP + 6 ml/l honey only need six days for shoot induction
INDUKSI TUNAS DARI EKSPLAN BIJI TANAMAN NYAMPLUNG (Calophyllum inophyllum L.) DENGAN PENAMBAHAN Benzylaminopurine (BAP) SECARA In Vitro
(2014-03-28) Imaniah, Muthia Rahmatul; Fatonah, Siti; Isda, Mayta Novaliza
Nyamplung (Calophyllum inophyllum) is economicaly and ecologicaly multifunctional useful plants, which has the potential to produce biofuels. This study aims to determine the optimum concentration of BAP in promoting the formation of shoot from nyamplung seed explants. This study uses the BAP treatment arranged in a randomized (RAK) which are 0, 0.5, 1, 2, 4 and 8 mg/l. Data was analyzed using ANOVA (Analysis of Variance) and if significant followed by DMRT (Duncan's Multiple Range Test) with a level of 5%. The results showed that the best shoot induction is seed explant planted on MS medium in addition of 8 mg/l BAP, with the percentage of shoots at 100% and resulted in the highest number of shoot 4.8
INDUKSI TUNAS DARI EKSPLAN BONGGOL PISANG UDANG (Musa acuminata Colla) SECARA IN VITRO PADA MEDIA MS DENGAN PENAMBAHAN BAP DAN KINETIN
(2016-01-30) Wati, Ririn Sari; Isda, Mayta Novaliza; Siti, Fatonah
Musa acuminata Colla has many adventages and unique characteristics, such as high calium content 400 mg and low calorie. Therefore, this species is potential to be widely cultivated that need the availability of the banana seedlings. The occurance of this species in Riau is very scarce, especially in Kampar district due to the conventional cultivation method, so that in vitro propagation is necessary. The most important step in the in vitro propagation is shoot induction. This research used a randomized block design (RBD), which included two groups of treatment i.e. the variation of BAP concentrations (2, 4, 6, 8 mg/l) and the the combination between BAP and kinetin concentrations (2 mg/l BAP+0,4 mg/l kinetin, 4 mg/l BAP+0,4 mg/l kinetin, 6 mg/l BAP+0,4 mg/l kinetin, 8 mg/l BAP+0,4 mg/l kinetin) on MS medium with 5 replications. The result showed that the addition of BAP and combination between BAP and kinetin were the best results with 100% explant growth and shoot induction. The group with 8 mg/l BAP + 0,4 mg/l kinetin showed only needs 10,40 hst for shoot induction.
INDUKSI TUNAS EKSPLAN BIJI TEMBESU (Fagraea fragrans Roxb.) PADA MEDIA WPM DENGAN PENAMBAHAN BAP SECARA IN VITRO
(2016-01-28) Haryono, Noto; Isda, Mayta Novaliza; Fatonah, Siti
One of the forest crop that has high prospect to be developed as quality for strong wood is tembesu (Fagraea fragrans Roxb.). Its wood can be used for a heavy construction and home construction. The seedlings produced from seeds of tembesu in natural stands are rarely found seedlings causing problems in propagation. The aim of this research was to determine the effect of BAP and the best concentration in inducing shoots from seed tembesu explants using in vitro method. This research was conducted at the Laboratory of Integrated Biology Department of Biology Faculty of Mathematics and Natural Sciences University of Riau from December to April 2015. This research used a Completely Randomized Design (CRD) with the concentration of BAP (0; 0,5; 1; 1,5; 2; 2,5; 3; 3,5; 4 mg/l) with 5 replications. Seeds the were planted in the media WPM for 120 days. The results of this research showed the highest percentage of shoot formation is 100% contained in the control treatment. The highest number of shoots produced in treatment of 1 mg/l BAP was 5,93 shoots. The highest average of shoot length was in control treatment 3,14 cm
MORFOLOGI TANAMAN DARI FAMILI TACCA (TACCACEAE)
(Elfitra, 2022-07) Habiba, Nur Hafsah; Isda, Mayta Novaliza
Plants belonging to the family Taccaceae are tropical plants that have a unique flower shape. Tacca flowers have unique accessories in the form of bracts and bracteoles. Information about the Tacca plant is very limited. In additon, this plant is difficult to find in its natural habitat. This study aimed to obtain information on the morphology of the Tacca plant. This study used samples from Harau Valley, West Sumatra, and were subsequently propagated by planting in the Botanical Laboratory of FMIPA UNRI. Morphological observations were made on stems, leaves and flowers. The results showed that the morphology of Tacca sp had light green stem, the tip and base of leaf were tapered (acuminate) and the leaf blade was lanceolate. Flowers of Tacca sp were dark purple with four purple-black bracts and yellowish-white bracteoles (whiskers) with wavy shapes.
Mulsa Organik Imperata cylindrica (L.), Mucuna Bracteata DC. Dan Kompos Pelepah Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Penghambatan Perkecambahan Dan Pertumbuhan Gulma Mikania micrantha H.B.K.
(2017-10-10) Fatonah, Siti; Malinda, Sari; Herman; Isda, Mayta Novaliza
Penelitian ini bertujuan untuk menentukan pengaruh ketebalan dan jenis mulsa (mulsa Imperata cylindrica, Mucuna bracteata dan kompos pelepah kelapa sawit) terhadap perkecambahan dan pertumbuhan gulma Mikania micrantha dalam polibag di kebun. Percobaan menggunakan Rancangan Acak Kelompok, dengan berbagai perlakuan tiga jenis mulsa baik tunggal maupun kombinasinya dengan ketebalan 2,5 dan 5 cm, yang terdiri dari 11 perlakuan. Masing-masing perlakuan diulang sebanyak tujuh ulangan. Hasil penelitian menunjukkan bahwa pemberian mulsa I. cylindrica, M. bracteata dan kompos pelepah kelapa sawit serta kombinasinya mampu menghambat perkecambahan dan pertumbuhan gulma M. micrantha. Hampir semua perlakuan mulsa mampu menghambat perkecambahan dan pertumbuhan gulma M. micrantha dengan penghambatan sebesar 100 %, kecuali perlakuan mulsa M. bracteata dengan penghambatan perkecambahan sebesar 97 %.
MULTIPLIKASI TUNAS IN VITRO DARI EKSPLAN NODUS JERUK SIAM (Citrus nobilis LOUR.) ASAL KAMPAR DENGAN PENAMBAHAN BENZYLAMINOPURINE (BAP) DAN EKSTRAK MALT
(2016-01-30) Rohmawati, Siti; Fatonah, Siti; Isda, Mayta Novaliza
Citrus nobilis Lour. from Kampar is a famous citrus plant in Riau province, that is highly demanded by many people in Riau. However, the plantation area of citrus decrease due to disease attack. In order to recover this condition, it is necessary to provide citrus seedlings in large quantities. The conventional propagation requires a lot of plants and takes a long time, therefore the in vitro propagation is necessary. This study aimed to find the effects and to determine best concentration BAP alone or in combination with malt extract to shoot multiplication nodal explants in vitro Citrus nobilis Lour. This experiment was designed using a randomized block design (RBD). The treatment was giving BAP alone or in combination with malt extract. The results showed that the best treatment for shoot multiplication was in MS medium suplemented with 0.5 mg / l BAP that produced the highest number of shoots (5 shoots per explant) with number of leaves was (2 leaves per eksplan). Supply malt extract was not goot to increase number of shoot.
Optimasi Konsentrasi Kanamisin pada Eksplan Kedelai (Glycine max L. Merr) Untuk Transformasi gen TcPIN
(2017-02-10) Isda, Mayta Novaliza
Increase of production soybean plagued by pod borer (Etiella zinckenella, Tr.). This pest is one factor that leads to lower soybean production. This pest can cause yield losses of up to 80% of soybean production. Until now there is no way of effective and efficient control of the pest is spreading very fast. One of the genes for traits such as plant resistance to pod borer is Proteinase Inhibitor (PIN) which is toxic to predators and pathogens. The TcPIN genes derived from cocoa crop This study aims to determine the concentrations of kanamycin prior to transformation TcPIN genes in soybean plants. Soybean explants grown in culture media that have been added in various concentrations of kanamycin. The results showed that the optimum concentration obtained kanamycin concentration of 50 ppm (mg / L).
PENGARUH ASAL BAHAN STEK DAN KONSENTRASI INDOLE BUTYRIC ACID TERHADAP PERTUMBUHAN STEK APEL INDIA (Ziziphus mauritiana Lam.)
(2020-05) Nurhikmah, Isti; Isda, Mayta Novaliza
Indian apples (Ziziphus mauritiana Lam.) is a fruiting plant originating from India. The spread of this plant can grow in subtropical and tropical regions. Generative propagation is difficult to carry out because Indian apple seeds are classified as hard-skinned seeds (physical dormancy). Vegetative propagation is an alternative to the multiplication of Indian apple seeds, through cuttings. Giving Indole Butyric Acid (IBA) as a type of synthetic auxin, has been proven to increase rooting in many plants. This study aimed to determine the effect of (IBA) as growth regulators with various concentrations on shoot, middle, and base cuttings using Completely Randomized Design with 8 treatments. The results obtained from this study stated that there was the effect of morphological changes in cuttings on the shoot middle, and base cuttings. The number and length of root were not produced in all treatments. Formation of nodules was only found in the stem cuttings with 21 (days after planting) nodules appearing at a concentration of 25 mg/L.
PENGARUH KONSENTRASI DAN INTERVAL WAKTU PEMBERIAN PUPUK ORGANIK CAIR NASA TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN TOMAT (Solanum lycopersicum Lam.)
(2013-07-19) Zabarti, Ella; Lestari, Wahyu; Isda, Mayta Novaliza
Tomato plant (Solanum lycopersicum Lam.) is one of the horticultural commodity with high economic value. Based on the tomato production data in Riau, the tomato productivity in 2010 in this province was lower than North Sumatra and West Sumatra, therefore the tomato production in Riau needs to be improved. One of the factors that can improve tomato production by fertilization. The application of fertilizer should be made more effectively with proper concentration and fertilizing time interval. One of liquid organic fertilizers that can be used to support the production of tomato plant is NASA liquid organic fertilizer. This fertilizer can be applied through its leaves. This study aims to determine the concentration and the fertilizing time interval of liquid organic fertilizer for optimum growth and production of tomato plant. This research had been conducted based on a factorial randomized block design, the first factor was the concentration and the second factor was the fertilizing time interval of NASA liquid organic fertilizer. Data were analyzed using ANOVA and followed by Duncan's New Multiple Range Test (DNMRT) at 5% significant level. The results showed that a single factor of concentration and the time interval of NASA liquid organic fertilizer did not gave a significant effect on the tomato growth and production. The interaction of treatments 8 cc/l NASA liquid organic fertilizer with 2 week interval gave a significant effect on the average tomato plant height (132.00 cm), but not on the other parameters, i.e. flowering date, number of flower per plant, number of fruit per plant and fruit weight per plant.
PENINGKATAN KUALITAS TANAH DAN PERTUMBUHAN TANAMAN CABAI MERAH (Capsicum annuum L.) DENGAN PEMANFAATAN CACING TANAH (Pontoscolex corethrurus Fr. Mull.)
(2014-05-22) Lestari, Wahyu; Isda, Mayta Novaliza
Ability of earthworm ( Pontoscolex Corethrurus Fr. Mull.) as degrade organic materials organism represent one of the alternative to get peaceful organic manure of environment and yield optimal material content. This research aim to know its ability in changing the nature of chemical physics of land and also see its influence to growth of red chilli (Capsicum annuum L.). As a whole result of research indicate that, earthworm exploiting and giving of livestock dirt at media plant, can denature land chemistry and physics so that can improve growth of red chilli.
Peningkatan Kualitas Tanah Dan Pertumbuhan Tanaman Cabai Merah (Capsicum annuum L.) Dengan Pemanfaatan Cacing Tanah (Pontoscolex corethrurus Fr. Mull.)
(2017-10-13) Lestari, Wahyu; Isda, Mayta Novaliza
Kemampuan cacing tanah (Pontoscolex corethrurus Fr. Mull.) sebagai organisme pengurai bahan organik merupakan salah satu alternatif untuk mendapatkan pupuk organik yang aman lingkungan dan meghasilkan kandungan hara yang optimal. Penelitian ini bertujuan untuk mengetahui kemampuannya dalam merubah sifat fisika kimia tanah serta melihat pengaruhnya terhadap pertumbuhan tanaman cabai merah (Capsicum annuum L.). Secara keseluruhan hasil penelitian menunjukkan bahwa, pemanfaatan cacing tanah dan pemberian kotoran ternak pada media tanam, dapat mengubah sifat fisika dan kimia tanah sehingga dapat meningkatkan pertumbuhan tanaman cabai merah.
PERBANYAKAN ANGGREK MACAN(Grammatophyllum scriptum (L.) Blume) SECARA IN VITRO DARI EKSPLAN TUNAS PADA MEDIA VACIN AND WENT
(2016-01-28) Susanto, Doni; Isda, Mayta Novaliza; Fatonah, Siti
Grammatophyllum scriptum (L.) Blume is an orchid species with threatened occurance in its natural habitat. In order to maintain the occurance of this species, it is necessary to conduct conservation efforts such as using in vitro culture technique. This study aimed to determine the best concentration of coconut water as well as its combination with BAP in the bud propagation of G. scriptum, using bud explants produced from in vitro culture. This study used a completely randomized design (CRD) with giving of various concentrations of coconut water treatment or in combination with BAP. The results showed that coconut water treatment or in combination with BAP could form buds and roots with a percentage of 100%. The coconut water treatment or in combination with BAP significantly affected the number of buds. The treatment of 1 mg/l BAP combined with 60% coconut water produced the highest number of shoots is (2.20).
PRODUKSI BIOFERTILIZER CAIR MENGGUNAKAN BAKTERI PELARUT FOSFAT ASAL TANAH GAMBUT RIAU YANG DIPERKAYA ZAT ADITIF
(2016-01-28) Putri, Ami Shafira; Zul, Delita; Isda, Mayta Novaliza
Efficiency of P chemical fertilizers absorption in peat lands can be increased by using phosphate - solubilizing bacteria (PSB). Phosphate solubilizing bacteria is one of soil microorganism group which is capable of dissolving the source of P that accumulates in the soil and turn it into an available form for plants. Because of its role, the PSB can be employed as biofertilizer agent. The objective of this study was to analysis shelf life of PSB in liquid biofertilizer amended with additive substances such as starch and tween + glycerol. Four potential PSB isolates were subcultured on Pikovskaya's slant agar. The isolates were cultivated on Pikovskaya's broth separately and then mixed them equally with a combination of two, three and four isolates resulting starter 1 (consist of BB_UB6, BB_K2, BB_K9 and BB_HS13), starter 2 (consist of BB_UB6, BB_K2 and BB_K9) and starter 3 (consist of BB_UB6 and BB_K9). The fermentation media were inoculated by those starters and also added with starch and tween + glycerol separately, then incubated for 4 days at room temperature to produce liquid biofertilizer. Biofertilizer were pack afterward and stored for 0, 30, 90 and 180 days in refrigerator. Each shelf life was analyzed by calculating the population of PSB and pH measurement. The addition of additive substances are generally able to maintain and even improve the stability of PSB populations in liquid biofertilizer until 180 days storage and bacterial cell number are still in the range of quality standards (1010 - 1011 CFU/ml). Liquid biofertilizer amended with additive substances still has good pH range for acidophilic PSB growth (4,1 - 5,6) until 180 days storage.
RESPON PERKECAMBAHAN BIJI TEMBESU (Fragraea fragrans Roxb.) PADA PERENDAMAN BERBAGAI KONSENTRASI GIBERELIN (GA3)
(2016-01-26) Normaya, Desva; Isda, Mayta Novaliza; Fatonah, Siti
Tembesu is a high quality wood crop. Tembesu seeds has natural dormancy because its small and hard seed. One way to break this dormancy is by soaking with giberelin (GA3) for 24 hours. This study used complete randomized design with five treatments i.e control, 50 mg/L , 75 mg/L , 100 mg/L and 125 mg/L giberelin concetration , with five replications each treatment. This research aimed to know the influence of various giberelin solutions and to determine the correct concentration for germination and growth process of tembesu. The result showed that all of the treatments could increase the germination of Tembesu seeds. The best giberelin concentration for the germination was 125 mg/L.

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