Induksi Tunas Dan Pembentukan Akar Dari Eksplan Kotiledon Jeruk Siam (Citrus Nobilis Lour.) Asal Kampar Secara In Vitro

Abstract

Tissue culture was an alternative way in micropropagation of siam orange (Citrus nobilis Lour.) from Kampar one of the main commodity in Riau province. Shoot induction as an early stage propagation and the rooting stage were very important parts to produce plantlet in vitro technique. The aim of this study was to determine the best BAP and NAA concentration for shoot induction and rooting of siam orange from Kampar. This study using a randomized block design with faktorial treatment. The results of this study show that the best shoot induction was obtained from cotyledon explant grown on MS medium without BAP. The percentage and the average number of shoot were 91,67% and 1,50 respectively. The combination of MS medium with 1 mg/L NAA was the most optimum concentration in rooting induction of siam orange from Kampar, with the percentage of root growth and the highest average number of root 90% and 6.3 roots respectively.

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Keywords

in vitro, Citrus nobilis Lour, BAP, NAA, cotyledon explant

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