INDUKSI TUNAS DARI EKSPLAN BONGGOL PISANG UDANG (Musa acuminata Colla) SECARA IN VITRO PADA MEDIA MS DENGAN PENAMBAHAN BAP DAN KINETIN
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Date
2016-01-30
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Abstract
Musa acuminata Colla has many adventages and unique characteristics, such as high calium content 400 mg and low calorie. Therefore, this species is potential to be widely cultivated that need the availability of the banana seedlings. The occurance of this species in Riau is very scarce, especially in Kampar district due to the conventional cultivation method, so that in vitro propagation is necessary. The most important step in the in vitro propagation is shoot induction. This research used a randomized block design (RBD), which included two groups of treatment i.e. the variation of BAP concentrations (2, 4, 6, 8 mg/l) and the the combination between BAP and kinetin concentrations (2 mg/l BAP+0,4 mg/l kinetin, 4 mg/l BAP+0,4 mg/l kinetin, 6 mg/l BAP+0,4 mg/l kinetin, 8 mg/l BAP+0,4 mg/l kinetin) on MS medium with 5 replications. The result showed that the addition of BAP and combination between BAP and kinetin were the best results with 100% explant growth and shoot induction. The group with 8 mg/l BAP + 0,4 mg/l kinetin showed only needs 10,40 hst for shoot induction.
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BAP, shoot induction, in vitro, kinetin, Musa acuminata Colla