Optimasi Konsentrasi Kanamisin pada Eksplan Kedelai (Glycine max L. Merr) Untuk Transformasi gen TcPIN
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Date
2017-02-10
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Abstract
Increase of production soybean plagued by pod borer (Etiella zinckenella, Tr.). This pest is one factor that leads to lower soybean production. This pest can cause yield losses of up to 80% of soybean production. Until now there is no way of effective and efficient control of the pest is spreading very fast. One of the genes for traits such as plant resistance to pod borer is Proteinase Inhibitor (PIN) which is toxic to predators and pathogens. The TcPIN genes derived from cocoa crop This study aims to determine the concentrations of kanamycin prior to transformation TcPIN genes in soybean plants. Soybean explants grown in culture media that have been added in various concentrations of kanamycin. The results showed that the optimum concentration obtained kanamycin concentration of 50 ppm (mg / L).
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TcPIN gene, soybean pod bore, proteinase, inhibitor, transformation, kanamycin