INDUKSI TUNAS DARI EKSPLAN BIJI MANGGIS (Garcinia mangostana L.) DIBELAH EMPAT PADA MEDIA MS (MURASHIGE-SKOOG) DENGAN PENAMBAHAN BAP DAN MADU SECARA IN VITRO
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Date
2016-01-28
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Abstract
Mangosteen (Garcinia mangostana L.) is a tropical plant that has export value
because it has a rich of antioxidant, xanthone, alpha and beta, that can be used for
medicine as an anti-cancer agent. One of the alternative method to produce large
number and uniform mangosteen seedlings inshort period can be done using in vitro
culture technique. The purpose of this research was to determine the optimal
concentration of BAP and honey in inducing shoot from of mangosteen explants
from seed that had been divided into four parts and also to determine the best
combination to induce mangosteen shoot uisngin vitromethod. The research was
conducted at Integrated Biology Laboratory Department of Biologi, Faculty of
Mathematics and Natural Sciences, Riau University from January until April 2015.
This research used a Completely Randomized Design (CRD) with the concentration
of BAP (3; 7 mg/l) and honey (3; 6; 9 ml/l), or in combination on MS medium with
5 replications.The result showed that the treatment on medium supplemented with
each BAP and honeyor in combination gave the highest percentage of explant
growthand shoot formation (100%)while the medium supplement with 7 mg/l BAP +
6 ml/l honey only need six days for shoot induction
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Keywords
Benzylaminopurin, Honey, In vitro, Shootinduction, Mangosteen (Garcinia mangostana L.)