Abstract:
Pyrazoline is a five-ring heterocyclic compound containing two nitrogen atoms in the
ring. Pyrazoline had a wide variety of biological activities, such as an inhibitor of the
tyrosinase enzyme. Pyrazoline 4-(3-(4-florophenyl)-5-(2-methoxyphenyl)-4-5-dihydro-
1H-pyrazole-1-yl)benzenesulfonamide has been successfully synthesized through
various stages. The first step is the formation of chalcone compounds by reacting 4-
floroacetophenone and 2-methoxybenzaldehyde through the Claisen-Schmidt reaction
using microwave irradiation. Then it is reacted with
4-hydrazinylbenzenesulfonamide which is synthesized through azotation and further
reduction reactions, then the cyclization reaction between chalcone and
4-hydrazinylbenzensulfonamide by reflux method for 9 hours at 80°C. The structure of
the synthesized compounds was confirmed by characterization using ultraviolet (UV)
spectroscopy, fourier transform infra red (FTIR) and high-resolution mass spectroscopy
(HRMS). The yield obtained from this pyrazoline synthesis was 93%. The toxicity of
pyrazoline compounds was tested using the Brine Shrimp Lethality Test (BSLT)
method using Artemia salina leach. The pyrazoline compound obtained was non-toxic
with an LC50 value of 3,706 μg/mL.
