ISOLASI DAN UJI AKTIVITAS PENGHAMBATAN α-GLUKOSIDASE SENYAWA METABOLIT SEKUNDER EKSTRAK n-HEKSANA DARI DAUN NONA MAKAN SIRIH (Clerodendrum thomsoniae Balf.f.)
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Date
2022-07
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Elfitra
Abstract
Clerodendrum thomsoniae is a species of the Lamiaceae family which is used as a
medicinal plant. Previous studies on several species of the genus Clerodendrum has
shown that this genus has secondary metabolites that have the potential to be
antidiabetic, antioxidant, anti-inflammatory, anticancer, and antiasma agents. This study
aims to isolate secondary metabolite compounds from the leaves of C. thomsoniae and
test the inhibitory activity of the enzyme α-glucosidase. Isolation of the plants leaves
has been carried out by the maceration method using methanol solvent and partioned
with n-hexane. The metabolites of n-hexane fraction have been separated using the
vacuum liquid chromatography method. Fraction 4 of VLC has been re-separated by the
flash chromatography method and obtained 93 vials. Purification of the compound was
carried out on vials 21-22 and white crystalline solids (10.75 mg) were obtained.
Characterization of the compound was carried out by FTIR spectroscopy which showed
the presence of OH, aliphatic C-H, CH2, CH3, C=C alkenes cyclic and C-O (alcohol)
groups. The melting point of the compound in the range of 140-142°C. The TLC profile
of CT-H-V4-FL21 was not similar to β-sitosterol, therefore these two compounds were
different. The enzyme inhibition test of α-glucosidase showed this compound had a
weak inhibitory activity with an inhibition percentage of 6.60%.
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Keywords
Clerodendrum thomsoniae, inhibition, isolation
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