Abstract:
Hydrazone which is a compound with the group ‒CH=N‒NH‒. Hydrazone containing
azomethin -NHN=CH- can be synthesized by heating at a certain temperature by
substituting hydrazine/hydrazide with an aldehyde and has various bioactivities such as
anti-cancer, anti-inflammatory, and anti-bacterial. For further development in this research,
the synthesis of the compound N'1N'3-bis-((E,Z)-2-bromobenzyliden)malonohydrazide was
carried out by reacted malonohydrazide with 2-bromobenzaldehyde added glacial acetic
acid catalyst to accelerate the formation reaction through a condensation reaction used
heating method (reflux) at temperature 50-60°C and stirring. The yield of the purity of the
compound was determined by using the TLC, melting point and HPLC analysis. The
purified compounds were identified using spectroscopic analysis of UV, and FTIR,.
Toxicity test was determined by BSLT method used shrimp larvae (Artemia salina Leach).
Based on the results of research that has been carried out, the DB-2Br compound has been
successfully synthesized with a yield of 90%, and is toxic with a value of LC50 = 2.8
μg/mL.
