PENGARUH pH DAN SUHU TERHADAP AKTIVITAS ENZIM LAKASE Trichoderma asperellum LBKURCC1 HASIL PENGENDAPAN 0-20% AMONIUM SULFAT

Abstract

Laccase Trichoderma asperellum LBKURCC1 an oxidoreductase enzyme with the ability to oxidize various phenolic groups using oxygen as an electron acceptor. Laccase activity can be affected by pH and temperature. In this study, the determination of fractionated laccase activity was investigated in order to obtain higher laccase activity than crude extract activity. Determination of laccase activity was carried out using a spectrophotometer with a wavelength of 420 nm. The results the highest activity was obtained at pH 5,5 and a temperature of 60⁰C.