Abstract:
Pyrazole is five ring heterocyclic compounds with two adjacent nitrogen atoms and it
have two endocyclic double bonds which is known to have diverse biological activities
such as anti-inflammatory, analgesic, antibacterial, antimalaria, antioxidant, and
anticancer. Pyrazole analogue 5-(4-fluorophenyl)-3-(naphthalen-2-yl)-1-phenyl-1Hpyrazole
(PZL-4F-AN2) was successfully synthesized through synthesis of one-pot
cyclization of pyrazoline which was then followed by oxidative aromatization reaction
of pyrazoline 5-(4-fluorophenyl)-3-(naphthalen-2-yl)-1-phenyl-4,5-dihydro-1Hpyrazole
with glacial acetic acid by refluxing the mixture for 36 hours in the oil bath at
85oC. The purity of the compound was determined by using TLC, melting point and
HPLC analysis. The structure was confirmed using UV and FTIR spectroscopy analysis.
The yield of the synthesis PZL-4F-AN2 compound was 18.29%. The toxicity of
pyrazole was evaluated by Brine Shrimp Lethality Test (BSLT) method with shrimp
larvae (Artemia salina Leach). Pyrazole PZL-4F-AN2 had high toxicity with LC50 =
43.07 μg/mL.
