OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH trnQ-5’ rps16 INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAU
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Date
2020-08
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Abstract
Pandan (Benstonea sp.) is one of the most important plant in Kajuik Lake located
in Langgam, Riau Province, Indonesia. Polymerase Chain Reaction (PCR) is a
technique used to multiply or amplify DNA samples in vitro using a primer pair.
trnQ-5’ rps16 intergenic spacer is a pair of primers used to amplify the trnQ-
5’ rps16 intergenic spacer region. This study aimed to optimize annealing
temperature of trnQ-5’ rps16 intergenic spacer primer. This research invloved
total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), PCR, and
electrophoresis. The result showed that the optimal annealing temperature for
amplification of trnQ-5’ rps16 intergenic spacer was 58°C. The PCR product on
this annealing temperature was thick DNA with a size of about 950 bp .
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Keywords
Annealing, Benstonea sp, PCR