OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH trnQ-5’ rps16 INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAU

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dc.contributor.authorNurhayati, Siti
dc.contributor.supervisorRoslim, Dewi Indriyani
dc.date.accessioned2021-07-29T07:07:55Z
dc.date.available2021-07-29T07:07:55Z
dc.date.issued2020-08
dc.description.abstractPandan (Benstonea sp.) is one of the most important plant in Kajuik Lake located in Langgam, Riau Province, Indonesia. Polymerase Chain Reaction (PCR) is a technique used to multiply or amplify DNA samples in vitro using a primer pair. trnQ-5’ rps16 intergenic spacer is a pair of primers used to amplify the trnQ- 5’ rps16 intergenic spacer region. This study aimed to optimize annealing temperature of trnQ-5’ rps16 intergenic spacer primer. This research invloved total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), PCR, and electrophoresis. The result showed that the optimal annealing temperature for amplification of trnQ-5’ rps16 intergenic spacer was 58°C. The PCR product on this annealing temperature was thick DNA with a size of about 950 bp .en_US
dc.description.sponsorshipDosen Bidang Genetika Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/10079
dc.language.isoenen_US
dc.subjectAnnealingen_US
dc.subjectBenstonea spen_US
dc.subjectPCRen_US
dc.titleOPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH trnQ-5’ rps16 INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAUen_US
dc.typeArticleen_US

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