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Item ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI AKTINOMISETES DARI ARBORETUM UNIVERSITAS RIAU(2017-01-10) Rahayu, Fatimah; Roza, Rodesia Mustika; Pratiwi, Nova WahyuDemand of antibiotics for phatogenic bacteria is getting increased, therefore, the discovery of novel antibiotics is needed to address antibiotics resistancy problem. Actinomycetes are well known to produce secondary metabolites such as antibiotics. Isolation of actinomycetes was conducted by making initial treatment of 4 soil samples with dry heat and wet heat to inoculate on Starch Casein Agar (SCA). From 4 sampled location at Univesitas Riau Arboretum, Location 3 had the most various of actinomycetes discovered. Wet heat treatment gave higher number of isolated actinomycetes with a number of 23 isolates. In this study, total actinomycetes isolated from samples were 30 isolates and tested their antibacterial activity. Actinomycetes isolate code C2.20 had the widest clear zone of 21.29 mm against pathogenic bacteria Staphylococcus aureus with agar disc method.Item POTENSI MIKROBA INDIGENUS ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU DALAM MENGHAMBAT PERTUMBUHAN Xanthomonas oryzae pv. oryzae(2014-03-28) Wizra, N.; Roza, R. M.; Martina, A.The plant disease caused by Xanthomonas oryzae pv. oryzae is still the major problem that can reduce agriculture productivity up to 20%. Currently the use of chemical bactericides can cause environment pollution and resistance to bacteria. Microorganisms can be used as biocontrol agent that is environmental friendly and potential to be developed. This study aimed to find the potential microbe from peat land at Rimbo Panjang village, Kampar Riau to produce antibacterial activity against Xanthomonas oryzae pv.oryzae. Antibacterial activity of microbe was tested using agar disk method and measured based on the ratio between the clear zone and colony diameter (ratio Z/K). The results showed 21 isolates of actinomycetes and 23 fungus isolates had antibacterial activity. The highest ratio activity for actinomycetes isolates was produced by Streptomyses RBIS6 (2.98) while the highest ratio for fungus isolates was produced by of Penicillium sp. L5J15 (2.00). Actinomycetes were the most potential microbes to produce antibacteria than the other microbes.