ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI AKTINOMISETES DARI ARBORETUM UNIVERSITAS RIAU
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Date
2017-01-10
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Abstract
Demand of antibiotics for phatogenic bacteria is getting increased, therefore, the
discovery of novel antibiotics is needed to address antibiotics resistancy problem.
Actinomycetes are well known to produce secondary metabolites such as antibiotics.
Isolation of actinomycetes was conducted by making initial treatment of 4 soil
samples with dry heat and wet heat to inoculate on Starch Casein Agar (SCA). From
4 sampled location at Univesitas Riau Arboretum, Location 3 had the most various of
actinomycetes discovered. Wet heat treatment gave higher number of isolated
actinomycetes with a number of 23 isolates. In this study, total actinomycetes isolated
from samples were 30 isolates and tested their antibacterial activity. Actinomycetes
isolate code C2.20 had the widest clear zone of 21.29 mm against pathogenic bacteria
Staphylococcus aureus with agar disc method.
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Keywords
actinomycetes, antibacterial, Universitas Riau Arboretum