Browsing by Author "Fibriarti, Bernadeta Leni"

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    AKTIVITAS FOSFATASE TANAH DAN TOTAL POPULASI BAKTERI PELARUT FOSFAT: DAMPAK ALIH FUNGSI LAHAN GAMBUT MENJADI PERKEBUNAN SAGU DI RIAU
    (2017-01-03) Handayani, Fitri; Zul, Delita; Fibriarti, Bernadeta Leni
    Sebagian besar lahan gambut di Riau telah diubah untuk kegiatan pertanian. Konversi lahan tersebut mengakibatkan perubahan jenis vegetasi yang mengganggu siklus P dalam tanah karena adanya perubahan populasi dan aktivitas mikroba. Tujuan penelitian ini adalah untuk menganalisis dampak konversi lahan gambut menjadi perkebunan sagu terhadap karakteristik fisika-kimia tanah, aktivitas fosfatase tanah, dan total populasi bakteri pelarut fosfat. Sampel tanah diambil dari tujuh lokasi yang berbeda di Desa Lukun, Kecamatan Tebingtinggi Timur, Kabupaten Meranti, Riau, yaitu hutan alam (HA), perkebunan sagu yang ditanam dengan umur 6 bulan (HSB), usia tanaman sagu 7 tahun yang bercampur dengan karet umur 5 tahun (HS+K), usia perkebunan sagu 20 tahun (HS 20 TH), usia perkebunan sagu 40 tahun (HS 40 TH), usia perkebunan sagu 80 tahun (HS 80 TH), dan usia perkebunan sagu 120 tahun (HS 120 TH). Karakter fisika-kimia tanah diukur dengan mengikuti metode standar. Aktivitas fosfatase tanah diukur dengan menggunakan metode kolorimetri dan total populasi bakteri pelarut fosfat ditentukan dengan menggunakan metode Total Plate Count. Karakter fisika-kimia tanah bervariasi dengan kisaran pH 5,63-6,50, suhu 28,25-31,25°C, kelembaban 58,75-86,25%, berat kering tanah 0,68-0,79 g, kandungan air tanah 21,00-32,00%, berat volume tanah 0,21 -0,27 g/cm3. Aktivitas fosfatase asam tertinggi ditemukan di HS 40 tahun (40,33 mol PNP/hr/g tanah) dan terendah di HSB (13,71 mol PNP/hr/g tanah). Total bakteri pelarut fosfat tertinggi pada HS 40 tahun (1,68×104 CFU/g tanah) dan terendah pada HSB (0,12×104 CFU/g tanah). Berdasarkan data, dapat disimpulkan bahwa konversi lahan gambut menjadi perkebunan sagu mempengaruhi aktivitas fosfatase asam, tetapi tidak mempengaruhi total populasi bakteri pelarut fosfat.
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    BIOMASSA FOSFOR MIKROBA DAN FOSFATASE TANAH GAMBUT DI SEMENANJUNG KAMPAR: ANALISIS DAMPAK ALIH FUNGSI LAHAN TERHADAP STATUS UNSUR FOSFOR
    (2014-03-28) Rahmila; Zul, Delita; Fibriarti, Bernadeta Leni
    Kampar Peninsula is one of the peat areas that has a high conservation value in Riau. Most of this areas have been converted into agriculture, plantations, and industrial plantation forests. This activity will reduce the amount of vegetation and affect microbial community composition and activity, which in turn will affect the P cycle in peatlands. This study aims to analyze the impact of land use to microbial P biomass and soil acid phosphatase activity. Soil samples were taken from three different land use systems, namely secondary forest, the 7-8 year old palm plantation and cassava fields. Microbial P biomass was quantified by using Chloroform Fumigation Extraction (CFE) method, while soil acid phosphatase activity was determined by using of colorimetric method. Microbial P biomass was high in the secondary forest site (274,27 μg P/g soil), and the lowest was in the cassava fields (72,68 μg P/g soil). Soil acid phosphatase was very active in the secondary forest site (40,58 μmolPNP/hours/g soil) and the lowest activity was found in the cassava fields (22,68 μmolPNP/hours/g soil). From the data, it can be concluded that land use affects the microbial P biomass and acid phosphatase activity in The Kampar Peninsula
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    BIOSORPSI LOGAM BERAT CADMIUM (Cd) MENGGUNAKAN BIOMASSA BAKTERI ASAM LAKTAT LOKAL RIAU
    (2019-11) Fibriarti, Bernadeta Leni; Fatzuarni, Rafika; puspitasari, Nuria
    Saat ini, polusi logam berat telah menjadi masalah serius, logam mencemari tanah, air, tanaman dan akhirnya masuk ke rantai makanan dan tubuh. Kadmium (Cd) termasuk dalam daftar sepuluh besar logam berat berisiko Baru-baru ini, beberapa penelitian telah menunjukkan bahwa strain BAL (Bakteri Asam Laktat) mampu menghilangkan logam berat bahkan dalam konsentrasi rendah dari media air dan juga tubuh manusia. Dalam penelitian ini, diuji kemampuan 2 strain bakteri asam laktat lokal dalam mengurangi Cd secara in vitro. BAL (Bakteri Asam Laktat) SB3 dan SB6 Riau Lokal dikultur dalam medium MRSB (de Man Rogose Broth) yang mengandung Cadmium 5,10 dan 50 ppm. Sisa konsentrasi kadmium dalam medium setelah 24 jam inkubasi diukur menggunakan Atomic Absorption Streptophotometry (AAS). Hasil penelitian menunjukkan bahwa isolat BAL SB3 mampu menyerap Cd pada konsentrasi 5, 10 dan 50 ppm dengan efisiensi biosorpsi berturut-turut 46,6%, 28,7% dan 26,7%, sedangkan SB6 dengan efisiensi biosorpsi adalah 36,5%, 24,9% dan 24,1%
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    ENUMERASI TOTAL POPULASI BAKTERI PENAMBAT NITROGEN (N) TANAH GAMBUT DI TELUK MERANTI
    (2014-03-28) Agustine, Metha; Zul, Delita; Fibriarti, Bernadeta Leni
    Teluk Meranti is one of the peatland ecosystems that most of the regions have been converted into oil palm plantations, industrial forest plantation, and settlement. Environmental conditions disrupted by the land conversions will affect the population, diversity, and activity of soil microbes. The objective of the study was to analyze the impact of land use changes to nitrogen fixing bacterial cells number. The nitrogen fixing bacterial cells number was calculated by using a Total Plate Count (TPC) method. The results showed that the physical and chemical characteristics of the soil ranged from 3,63- 4,00 for pH, temperature 27oC-31,5°C, dry weight 14,35-29,70%, water content 70,30- 85,70% and bulk density 0,15-0,39g/cm3. The nitrogen fixing symbiotic bacteria cells number ranged from 3,6-10,0·104 CFU/g soil and the nitrogen fixing nonsymbiotic bacteria cells number ranged from 0,78·10-1,8·104 CFU/g soil. Based on the results obtained, it can be concluded that the soil quality in the Teluk Meranti is still capable enough to support the sustainability of soil biological processes
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    ISOLASI BAKTERI PATOGEN Ralstonia solanacearum DARI TANAMAN CABAI RAWIT (Capsicum frutescens) DI PERTANIAN CABAI TANAH MERAH
    (Elfitra, 2023-06) Utami, Metri Laksita; Fibriarti, Bernadeta Leni
    Ralstonia solanacearum is one of the bacteria that attacks chili plants which causes wilt disease. Chili plants attacked by R. solanacearum will be difficult to be recovered. R. solanacearum is a pathogenic bacterium which is transmitted through the soil. This bacteria is also one of the factors reducing chili yield in Indonesia. Cayenne pepper (Capsicum frutescens) in Tanah Merah chili farming is often attacked by wilt disease, so R. solanacearum isolation was carried out to identify bacterial wilt in chili farming in Tanah Merah and to obtain a collection culture so that further testing could be performed. R. solanacearum in this study was isolated from stems of the chili plant. Isolation of this bacteria using casamino acid peptone glucose medium. Then it was identified by observing the macroscopic and microscopic morphological characters. The results of this study obtained bacterial isolates with characteristics of R. solanacearum.
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    ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT (BAL) KANDIDAT PROBIOTIK DARI TERASI TRADISIONAL DI PEKANBARU
    (wahyu sari yeni, 2019-01-14) Ansumar, Ansumar; Fibriarti, Bernadeta Leni
    Terasi is one of the fermented product of shrimp or fish which is often used as food seasoning by the people. Terasi is also a source of Lactic Acid Bacteria (LAB) which has the potential as a culture for probiotic starters. Probiotics are dietary supplements containing live microbes that profitable to their hosts. This study aimed to characterize probiotic lactic acid bacteria isolated from traditional terasi in Pekanbaru. LAB isolation from terasi was done by pour plate method using selective medium, MRSA (Man Rogosa Sharpe Agar). All of morphological, physiological and biochemical characteristics were observed. A total of 7 isolates were obtained from the isolation process. All LAB isolates are rod (Basil) positive Gram bacteria, negative catalase, non-motile and capable to fermenting glucose. In the probiotic test seven isolates was able to grow at a temperature of 37 ℃ and 45 ℃, pH 2 and 3, and salt levels of 4% and 6.5%.
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    ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PENGHASIL EKSOPOLISAKARIDA DARI NIRA TEBU (Saccharum officinarum L.)
    (Elfitra, 2023-06) Ain, Ecce Azzahra; Fibriarti, Bernadeta Leni
    Lactic acid bacteria (LAB) are bacteria that are safe for human consumption. Some types of LAB are able to produce secondary metabolites including exopolysaccharides (EPS). EPS-producing LAB has been isolated from various ingredients that contain high sugar sources so that it can be found in sugarcane sap (Saccharum officinarum L.). This study aimed to isolate and characterize EPS-producing LAB from sugarcane sap. The isolates of LAB were obtained using the dilution method on MRSA medium (deMan Rogosa and Sharpe Agar) containing CaCO3, then characterized macroscopic, microscopic, biochemical and physiological properties. The results of the study obtained NT2 isolates that have gram-positive, the form of cocci cells, cannot produce indole, are able to ferment glucose and sucrose, negative catalase and non-motile.
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    ISOLASI, SELEKSI DAN UJI AKTIVITAS LIPASE BAKTERI LIPOLITIK ASAL LIMBAH CAIR INDUSTRI KELAPA SAWIT SEBAGAI PENYUSUN BIODETERGEN
    (Elfitra, 2022-08) Haura, Sari Rakha Al; Fibriarti, Bernadeta Leni
    Lipolytic bacteria are bacteria that produce lipase enzymes to hydrolyze fats into fatty acids and glycerol. Lipolytic bacteria can be found in oil-contaminated habitats such as palm oil mill effluent. One of the applications of lipase enzymes is as biodetergent formulations that are eco-friendly. This research aimed to obtain potential lipolytic bacteria isolates from palm oil mill effluent and analyze the potential of their lipase to be applied as a biodetergent formulation through several tests, namely enzyme activity test, enzyme activity stability test and washing test. The results obtained 22 isolates of lipolytic bacteria with one isolate of potential lipolytic bacteria. LSSB 4.1 isolate produced lipase activity of 4.125 U/ml, produced a relative activity of 88% when the isolate was exposed to 1% detergent and could remove oil from cotton fabric with a percentage of 61.3%. Lipase from LSSB 4.1 isolates has the potential to be developed as a biodetergents formulation.
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    KARAKTERISTIK MIKROBIOLOGIS MINUMAN FERMENTASI BUNGA ROSELLA (Hibiscus sabdariffa L.) MENGGUNAKAN BAKTERI ASAM LAKTAT LOKAL RIAU
    (Elfitra, 2023-04) Astari, Rachmi Yunisa; Fibriarti, Bernadeta Leni
    Probiotic drinks that are often found in the market are probiotic drinks made from milk, so it is necessary to use other alternative materials such as vegetable materials as raw materials for making probiotic drinks such as Rosella flowers (Hibiscus sabdariffa L.) to obtain probiotic drinks that are more affordable for the community and multifunctional. The purpose of this study was to produce fermented beverages from Rosella flowers fermented using Riau local lactic acid bacteria (LAB) JN2 and JN6. The microbiological characteristics test of Rosella extract fermented beverage included pH measurement parameters, total lactic acid, antioxidant activity with DPPH method, total lactic acid bacteria and detection test of pathogenic bacteria Salmonella sp. and Escherchia coli. The results obtained the best microbiological characteristics were P2 (Rosella extract + LAB JN6) with pH ranging from 1.40 ± 0.10 - 2.30 ± 0.43, the highest total lactic acid of 0.100 ± 0.00%, antioxidant activity of 12.5%, total lactic acid bacteria of 72 x 102 CFU/mL, and the absence of pathogenic bacteria Salmonella and Escherchia coli in Rosella extract fermented beverages. Making fermented beverages of dried Rosella flower extracts using Riau local lactic acid bacteria JN2 and JN6 cannot be utilized as probiotic bacteria for Rosella extract fermented beverages because the pH of the beverage is very acidic which makes LAB JN2 and JN6 difficult to grow.
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    POPULASI MIKROBA DI KAWASAN LAHAN GAMBUT YANG DIALIH FUNGSI MENJADI HUTAN TANAMAN INDUSTRI YANG DITANAMI Acacia crassicarpa
    (2017-01-03) Hidayah, Wardatul; Zul, Delita; Fibriarti, Bernadeta Leni
    Alih fungsi lahan gambut menjadi lahan pertanian atau hutan tanaman industri (HTI), mengakibatkan terganggunya mikrohabitat tanah dan pada akhirnya akan berdampak terhadap populasi dan aktivitas mikroba tanah. Aktivitas dan populasi merupakan indikator sensitif terhadap stres lahan, sehingga dapat digunakan untuk memantau kualitas dari ekosistem tanah. Penelitian ini bertujuan untuk menganalisis dampak dari alih fungsi lahan gambut menjadi HTI yang ditanami Acacia crassicarpa melalui karakterisasi fisika-kimia tanah dengan metode standar dan penghitungan total populasi bakteri kopiotrof, oligotrof, dan jamur dengan metode Total Plate Count (TPC). Sampel tanah diambil dari 3 lokasi yang berbeda yaitu area konservasi sebagai kontrol, akasia rotasi III, dan akasia rotasi IV. Hasil penelitian untuk pH, temperatur tanah, berat kering tanah, kandungan air, dan berat volume tanah berturut-turut berkisar antara 5,08-6,11, 28,54-300C, 0,62-0,72 g, 28-38%, dan 0,39-0,42 g/cm3. Total populasi bakteri kopiotrof berkisar antara 0,90-4,20 x109 CFU/g tanah. Total populasi tertinggi bakteri kopiotrof terdapat di lokasi HTI rotasi III, dan terendah terdapat di area konservasi. Total populasi bakteri oligotrof berkisar antara 0,74-4,53x109 CFU/g tanah. Total populasi bakteri oligotrof tertinggi terdapat di lokasi HTI rotasi III, dan terendah terdapat di area konservasi. Total populasi jamur berkisar antara antara 0,41-1,17x104 CFU/g tanah. Total populasi jamur tertinggi terdapat di area konservasi, dan terendah terdapat di HTI rotasi IV. Hasil penelitian menunjukkan bahwa dampak alih fungsi lahan berpengaruh terhadap total populasi bakteri kopiotrof, oligotrof, dan jamur.
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    SELEKSI BAKTERI ASAM LAKTAT LOKAL PENGHASIL EKSOPOLISAKARIDA DAN OPTIMASI PRODUKSI PADA VARIASI SUHU, WAKTU INKUBASI SERTA MEDIUM FERMENTASI
    (Elfitra, 2022-01) Lestari, Defy Indah; Fibriarti, Bernadeta Leni
    Exopolysaccharides (EPS) are secondary metabolites produced by microorganisms, EPS is produced by lactic acid bacteria (LAB) under unfavorable conditions. The purposes of this study were to select LAB capable of producing EPS and to determine the optimal time, temperature, and medium required by LAB to produce EPS. This study used 5 isolates, JN2, JN5, JN6, S3PP8, and S3PP12. In the selection stage of LAB producing EPS in 2% skim milk liquid media, the five isolates did not produce EPS. Selection results using MRSA added with sucrose showed that 1 isolate (JN6) produced mucoid EPS, while 4 isolates (JN2, JN5, S3PP8 and S3PP12) produced mucoid and ropy type EPS. EPS production test of S3PP12 isolates on MRSB-glucose and MRSB-sucrose medium showed that LAB could not produce EPS.
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    SELEKSI KEMAMPUAN BAKTERI PELARUT FOSFAT ASAL BUKIT BATU-RIAU DALAM MENGHASILKAN ASAM SIANIDA
    (2014-03-28) Aprillia, Pristiana; Zul, Delita; Fibriarti, Bernadeta Leni
    Phosphate solubilizing bacteria (PSB) is beneficial bacteria which are not only capable in solubilizing organic phosphate into inorganic phosphate, but also producing cyanide acid (HCN). HCN plays a role in controling weed growth. The objective of this research was to screen the potency of indigenous PSB isolated from peat soil in producting HCN. The collections of PSB were subcultured in King’B broth. The ability of 152 isolates on HCN production were tested qualitatively using filter paper soaked with picric acid and Na2CO3 . The result indicated that as many as 26 isolates (17.1%) were able to release various levels of HCN production based on the appearing of brown, dark brown, or reddish brown colour on the filter paper
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    TOTAL POPULASI MIKROBA DAN AKTIVITAS PROTEASE PADA TANAH GAMBUT DI CAGAR BIOSFER GIAM SIAK KECIL - BUKIT BATU RIAU
    (2013-03-04) Marliyana, Siti; Zul, Delita; Fibriarti, Bernadeta Leni
    Giam Siak Kecil-Bukit Batu (GSK-BB) Biosphere Reserve is one of peatland areas located in Riau Province that consist of conserved area and productive area. Most of these areas have been changed as oil palm plantation, industrial forest plantation, agriculture, and settlement. This research aim was to analyze land use change affect to microbial cells in GSK-BB Biosphere Reserve by analyzing physical and chemical characteristics of soil, quantifying microbial cells and determinating of protease activity. Physical and chemical characteristics of soil were measured by employing standard methods, microbial cells was quantified by using of total plate count method and protease activity was measured by using colorimetric method. Soil samples were taken from six different locations, namely primary forests (as a control), secondary forest, 14 years old rubber plantation, 40-60 years old rubber forests, 12 years old palm oil plantation, and 3 years old oil palm plantation. Soil physical and chemical characteristics varied in every location ranging from 3,5 for soil pH; temperature 28,25- 31,25oC; dry weight 14,70-34,70%; bulk density 0,08-0,38 g/cm3 and soil conductivity 8,75-20,5 me/100 g. Copiotropic bacterial cell number ranges from 7,05±0,85x104- 44±6,48x104 CFU/g soil. Oligotropic bacterial cell number ranges from 10,5±0,57x104-40,0±2,16x104 CFU/g soil. Proteolytic bacterial cell number ranges from 0,5±0,08x104 -4,6±0,53x104 CFU/g soil. Fungal cell number ranges from 2,42±0,78x103- 16,0±2,94x103 CFU/g soil. Actinomycetes cell number ranges from 4,00±1,15x103 - 9,25±1,70x103 CFU/ g soil. Protease activity ranges from 2,28-5,78 g tyrosine/ g soil/ hour. The results of this research show that land use changes influence the dry weight, bulk density, soil conductivity and microbial cells number.
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    UJI AKTIVITAS BAKTERI LOKAL PENGHASIL LIPASE DENGAN MENGGUNAKAN INDUSER MINYAK JAGUNG
    (Elfitra, 2022-07) Juniarti, Tiya; Fibriarti, Bernadeta Leni
    Lipase is an enzyme that is widely used commercially in industry, biotechnology and health fields. Lipase enzyme functions to hydrolyze oils and fats by breaking them down into short-chain lipids and fatty acids. Lipase enzyme from bacteria is the most widely used because it has advantages such as being easy and fast to grow, cultivated, widely distributed so that it is easy to isolate anywhere and is relatively cheaper. This study aimed to determine the effect of corn oil inducer on the activity of lipase producing bacteria. The research method was carried out quantitatively using the titration method. The results showed that the highest lipase activity value using corn oil as inducer was in BPT6 isolate with a lipase activity value of 1.52 U/ml, in BP6 isolate with a lipase activity value of 1.37 U/ml. The lowest lipase activity value was demonstrated by isolate BP1 with 0.85 U/ml at 24 hours of incubation.
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    UJI KEMAMPUAN BAKTERI LOKAL RIAU DALAM MENDEGRADASI KANTONG PLASTIK HITAM
    (Elfitra, 2023-06) Siregar, Elsa Novita Br; Fibriarti, Bernadeta Leni
    One of the most commonly found organic waste is plastic bags. Plastic is an inorganic waste that has constituent components that are difficult to decompose in nature. The use of microorganisms such as bacteria and the use of enzymes is an effective and environmentally friendly way. The purpose of this study was to determine the ability of Riau local isolate bacteria in degrading black High Density Polyethylene (HDPE) plastic bags. Rejuvenation of bacterial isolates is carried out to ensure the isolates used are appropriate. The biodegradation test was based on the percentage weight reduction of HDPE plastic sheets on the medium for 60 days. The results showed that the isolates had HDPE biodegradation activity that was able to degrade black plastic within 60 days incubation time. The percentage of weight reduction of the test plastic was by LSSB 2.3 isolate of (0.35%).
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    UJI POTENSI BAKTERI SELULOLITIK ASAL TANAH GAMBUT DI CAGAR BIOSFER GIAM SIAK KECIL-BUKIT BATU DALAM MELARUTKAN FOSFAT
    (2016-05-19) Monisyah, Sally; Zul, Delita; Fibriarti, Bernadeta Leni
    Giam Siak Kecil-Bukit Batu (GSK-BB) Biosphere Reserves is one of peatland area in Riau Province. Peatlands is characterized by its high content of organic matter, but has very low availability of macro elements, such as nitrogen, potassium, and micro elements, such as phosphorus. Phosphorus (P) is one of the important nutrients that play a role in the metabolism of soil and microbiological processes. In the soil, phosphor was found in the form of phosphate that couldn’t be utilized. One way to overcome this problem is to use phosphate-solubilizing bacteria (PSB). So far, there is no information about the ability of cellulolytic bacteria originated from GSK-BB Biosphere to dissolve phosphate. Therefore, it is necessary to study the potential cellulolytic bacteria in dissolving phosphate. Potential test was conducted using cellulolytic bacteria grown on pikovskaya. There were 57 isolates which is able to grow and forming a clear zone out of 61 cellulolytic isolates which were subcultured on pikovskaya media. The test showed that as many as 14 isolates (24,6%) have a high potential in dissolving phosphate. Based on the ratio of the diameter of clear zone and colony diameter, isolates K.2.2.10-2 showed the highest activity with a value ratio of 10,66.
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    UJI POTENSI KELARUTAN BATUAN FOSFAT OLEH BAKTERI PELARUT FOSFAT ASAL TANAH GAMBUT DI RIAU
    (2014-03-28) Novianti, Pradita Eka; Zul, Delita; Fibriarti, Bernadeta Leni
    Phosphate (P) is a macro nutrient needed by plant. In peat soil, P element usually becomes inorganic phosphate in mineralization process by the help of phosphate solubilizing microbes (PSM). Inoculation of phosphate solubilizing bacteria (PSB) into soil could be served as alternative biofertilizer. The purpose of this study was to examine the potential of 198 PSB which were isolated from peat soil in Bukit Batu and Kampar Peninsula, Riau Province in solubilizing rock phosphate. The ability of bacteria in rock phosphate solubilizing was determined quantitatively. A number of 198 PSB isolates which were inoculated to modified liquid pikovskaya’s medium consists of rock phosphate were categorized high, medium and low. The result of median value test on modified liquid pikovskaya’s medium which consists of rock phosphate reveals that 56 (28,28%) PSB isolates were categorized high with P concentration ≥1,17 mg/l