UJI AKTIVITAS BAKTERI LOKAL PENGHASIL LIPASE DENGAN MENGGUNAKAN INDUSER MINYAK JAGUNG
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Date
2022-07
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Elfitra
Abstract
Lipase is an enzyme that is widely used commercially in industry, biotechnology and
health fields. Lipase enzyme functions to hydrolyze oils and fats by breaking them down
into short-chain lipids and fatty acids. Lipase enzyme from bacteria is the most widely
used because it has advantages such as being easy and fast to grow, cultivated, widely
distributed so that it is easy to isolate anywhere and is relatively cheaper. This study aimed
to determine the effect of corn oil inducer on the activity of lipase producing bacteria.
The research method was carried out quantitatively using the titration method. The results
showed that the highest lipase activity value using corn oil as inducer was in BPT6 isolate
with a lipase activity value of 1.52 U/ml, in BP6 isolate with a lipase activity value of
1.37 U/ml. The lowest lipase activity value was demonstrated by isolate BP1 with 0.85
U/ml at 24 hours of incubation.
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Keywords
bacteria, corn oil, lipase enzyme, lipase activity
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