Browsing by Author "Balatif, N."

Now showing 1 - 11 of 11
  • No Thumbnail Available
    Item
    ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL DARI DAUN TANAMAN Annona squamosa L
    (2013-03-09) Amalia, R.; Teruna, H. Y.; Balatif, N.
    An investigation on the antioxidant activity of Annona squamosa extract and essential oil has been done using DPPH ( 1,1- diphenyl – 2 – picryl hydrazyl) method. The extract was obtained by macerating the leaves of Annona squamosa using n-hexane and methanol respectively. The methanol extract was fractionated by vacuum liquid chromatography, resulted essential oil fractionation (Fraction 4). The antioxidant activity of the extracts and essential oil fraction was expressed as percent inhibition of DPPH radical (IC50). The results showed that the methanol extract was more active (IC50 = 52.65) compared to n-hexane extract (IC50 = 470.17) while the essential oils have the lowest antioxidant activity (IC50 = 479.48). Analysis of chemical components in the essential oil of the leaves has been determined using Gas Chromatography-Mass Spectroscopy (GC-MS) method. The main components of the essential oil are E-2- octadecadecen-1-ol; methyl palmitate; n-hexadecanoic acid; methyl 11,14,17- eicosatrienoate; and muurolol with the percentage yield according to the peak height serially 26,66%, 16,35%, 8,66%, 8,13% and 5,94%
  • No Thumbnail Available
    Item
    ISOLASI DAN UJI TOKSISITAS EKSTRAK METANOL DARI DAGING BIJI TANAMAN SIRSAK (Annona muricata Linn)
    (2013-07-20) Murpratiwi, M.; Yuharmen; Balatif, N.
    Isolation and toxicity test of soursop (Annona muricata L) seed flesh have been done. The isolation of chemical compounds was done by maseration using n-hexane and methanol while its toxicity was determined by Brine Shrimp Lethality Test method using Artemia salina Leach. Gas Chromatography Mass Spectroscopy (GC-MS) was used to analyze the chemical compounds from methanol fraction. A total of 1100 g soursop seed flesh yielded 140.8 g of n-hexane extract and 108.6 g of methanol extract. The results of toxicity test showed that n-hexane extract has no toxicity (LC50 = 1122.02 ppm), while methanol extract has toxicity (LC50 = 0.59 ppm). The result of GC-MS analysis showed that the 6th fraction of methanol extract contained methyl palmitic (peak of 1.96 on 20.95 minutes retention time), methyl linoleic (peak of 2.12 on 22.99 minutes), and methyl oleic (peak of 2.23 on 23.05 minutes) based on NIST and WILEY 7 library.
  • No Thumbnail Available
    Item
    ISOLASI METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK METANOL DAUN TANAMAN SRIKAYA (Annona squamosa Linn)
    (2013-04-17) Tripiana, M.; Teruna, H. Y.; Balatif, N.
    An investigation on the toxicity of srikaya (Annona squamosa ) extract has been done by using the Brine Shrimp Lethality Test (BSLT). The extract was obtained by macerating the leaves of A. squamosa using n-hexane and methanol respectively. The methanol extract was fractionated by vacuum liquid chromatography method (VLC). The results showed that the extracts of n-hexane and methanol more toxic than the fraction 4.
  • No Thumbnail Available
    Item
    KUANTIFIKASI TOTAL MIKROBA INDIKATOR PADA DAERAH ALIRAN SUNGAI SIAK PERAWANG
    (2014-03-27) Ridho, M. R.; Jose, C.; Balatif, N.
    Drainage basin of Siak River in Perawang, a village in the district of Siak, is one of the downstream areas. It is a severe drainage basin which is indicated by the decreasing quality of Siak River that has already exceeded the standard indicator of sustainable river. This research aims to analyze the water quality of Siak River using microbiological parameters (microbial contamination indicators). The parameters applied are based on the standard quality of water according to Government Regulation No. 82 Year 2001. The water samples from drainage basin of Siak River were collected in July 2013 from four sampling sites (9 , 10 , 11 and 12). Microbial contamination indicators such as fungi, Coliform, and E. coli were determined by total plate count and total bacteria by Most Probable Number (MPN). The results showed that sampling site 11 had relatively higher number of total plate count and MPN, while the sampling site 10 was relatively lower and significantly different in total plate count and MPN compared to the other sampling sites. Based on Government Regulation, the results of the research showed that the microbiological parameters exceeded the standard quality
  • No Thumbnail Available
    Item
    SINTESIS (E)-1-NAFTALEN-1-IL-3-(3-NITRO-FENIL)-PROPENON DARI 1-ASETILNAFTALEN DAN 3-NITROBENZALDEHID
    (2014-03-27) Andriyani, N. C.; Balatif, N.; Jasril
    Chalcone of (E)-1-naphthalene-1-yl-3-(3-nitro-phenyl)-propenone has been synthesized by aldol condensation using NaOH as a catalyst. The synthesis of chalcone compound used stirring method by reacting 1-acetylnaphthalene with 3-nitrobenzaldehyde. (E)-1-naphthalene-1-yl-3-(3-nitro-phenyl)-propenone compound obtained has a good yield 88,68 %. The compound has been characterized using UV, FTIR, NMR and Mass spectroscopy. Data characterization showed that the compound obtained was target compound.
  • No Thumbnail Available
    Item
    SINTESIS (E)-3-(3-BROMOFENIL)-1-(NAFTALEN-2-IL)PROP-2-EN-2-ON DARI 2-ASETILNAFTALEN DAN 3-BROMOBENZALDEHID
    (2014-03-27) Muttakin, I.; Balatif, N.; Jasril
    Chalcone of (E)-3-(3-bromophenil)-1-(naphtalen-2-yl)prop-2-en-2-one has been synthesized from 2- acethylnapthalene and 3- bromobenzaldehyd by aldol condensation using the stirring method with base catalyst (NaOH). The yield obtained for this compound was 61.21 %. The purity of product has been evaluated using TLC, melting point test, and HPLC analysis. The structure of the synthesized compound was confirmed by UV UV, FTIR, NMR, and MS analysis. Data characterization showed that the compound obtained was the targeted compound
  • No Thumbnail Available
    Item
    SINTESIS (E)-3-(3-KLOROFENIL)-1-(NAFTALEN-1-IL) PROP-2-EN-1-ON DARI 1-ASETILNAFTALEN DAN 3-KLOROBENZALDEHID MELALUI KONDENSASI ALDOL
    (2014-03-27) Anisah; Balatif, N.; Jasril
    Chalcone (E)-3-(3-chlorophenyl)-1-(naphthalen-1-yl)prop-2-en-1-one was synthesized by aldol condensation reaction from 1-acetylnaphthalen with 3-chlorobenzaldehyde using NaOH catalyst by stirring method. (E)-3-(3-chlorophenyl)-1-(naphthalen-1- yl)prop-2-en-1-one compound obtained in yield 87,81 %. The structures of synthesized compounds were well characterized by UV, IR, NMR, and Mass spectroscopy showed that the compound obtained was correlated to our target compound
  • No Thumbnail Available
    Item
    SINTESIS DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA KURKUMIN (3E,5E)-3,5-BIS(2’-HIDROKSIBENZILIDIN)-1-METILPIPERIDIN-4 -ON
    (2014-03-27) Hasanah, U.; Zamri, A.; Balatif, N.; Eryanti, Y.
    The derivatives of curcumin are second ary metabolites belonging to the phenolic group. Some of them were reported to have biological activities such as anti -i nfl ammatory, anticancer and antioxidant activities. In this study, acurcumin of (3E,5E)-3,5bis(2’-hidroxybenzilidin)-1-metilpiperidin-4-one from 1-methyl-4-pi peridinone with 2-hydroxy benzaldehideh as been successfully synthesized using microwave irradiation method with NaOH (20 %) as catalyst . React ion was carried out for 1 minute at 180 watt . The compound resulted was in the form of yellow crystal withayield of 72 %. The compound was characterized by measuring its melting point, identifying by TLC, and elucidated by UV-Vis, FT- IR, MS, 1 H NMR, and 13 C NMR. Our result show edthatthecom pound had the target edmolecule structure. Ant i bacterial test was carried out using the agar di ffusion method against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Salmonella enteritidis. Overall, inhibition zone ran ged from 6.5 t o 10 mm and showed avery weak activity.
  • No Thumbnail Available
    Item
    SINTESIS DAN UJI TOKSISITAS SENYAWA KALKON (E)-1-(2- HIDROKSIFENIL)-3-(4-METOKSIFENIL)PROP-2-EN-1-ON
    (2014-03-27) Sofiana, A.; Balatif, N.; Zamri, A.
    Chalcone of (E)-1-(2-hydroxyphenyl)-3-(4-methoxyphenyl)prop-2-en-1-one has been synthesized by stirring 2’-hydroxyacetophenon with methoxybenzaldehide derivatives in polyethylene glycol (PEG-400) at room temperature according to Claisen-Schmidt condensation. In this research KOH is used as catalyst. The rendement obtained was 76% and the structure was confirmed by UV, IR, 1H-NMR and MS spectroscopy. The toxicity was evaluated by Brine Shirmp Lethality Test (BSLT) method againts larvae of Artemia salina Leach. The chalcone had good toxicity activity since its LC50 was 0.666 μg/m
  • No Thumbnail Available
    Item
    SINTESISDANUJITOKSISITASANALOG3’-BROMOKALKON
    (2014-03-27) Rahmadhona, S.; Balatif, N.; Zamri, A.
    Chalconeof1-(3’-Bromofenil)-3-(4’-Klorofenil)-2 propen-1-on has beensynthesized from3’-bromoasetofenonbystirrermethodandevaluatedfor its toxicity activity. 1-(3’-Bromofenil)-3-(4’-Klorofenil)-2-propen1-onshowedagoodyield, andthen it was characterizedusingUVspectroscopy, IR and 1 H-NMR. Its toxicity activityhasbeen identified usingBrineShrimpLethalityTest(BSLT)againstlarvae ofArtemiasalina Leach. The toxicity value of 1-(3’-Bromofenil)-3-(4’-Klorofenil)-2-propen-1-on obtainedwas1,03mg/ mL. It indicated that the chalconehadagoodtoxicity and potentialasanticancer.
  • No Thumbnail Available
    Item
    SINTESISDANUJITOKSISITASSENYAWAANALOG 3’,4’-DIMETOKSIKALKON
    (2014-03-27) Novianti, S.; Balatif, N.; Zamri, A.
    Chalconesbelongto important classofflavonoid compounds.Theyhavealsobeen reported to have anticancer, antimicrobial, antifungal, antioxidant, and cytotoxic activities. In this research, an analogous chalcone of(E)-3-(4clorophenyl)-1-(3’,4’ dimetoxyphenyl) prop- 2-en-1-on wassynthesized using microwaveiradiation and natriumhidroxideas catalyst. Thecompound showed a goodyield,and then was characterizedbyUVspectroscopy,IR, 1 H-NMR,andMassSpectroscopy.Its toxicity hasbeenidentified usingBrineShrimpLethalityTest(BSLT) methodonArtemia salina Leach. The result showed that it had a good toxicity activity with LC50=2.24µg/mLandpotentialasanticancer.