ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL DARI DAUN TANAMAN Annona squamosa L
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Date
2013-03-09
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Abstract
An investigation on the antioxidant activity of Annona squamosa extract and essential oil has been done using DPPH ( 1,1- diphenyl – 2 – picryl hydrazyl) method. The extract was obtained by macerating the leaves of Annona squamosa using n-hexane and methanol respectively. The methanol extract was fractionated by vacuum liquid
chromatography, resulted essential oil fractionation (Fraction 4). The antioxidant
activity of the extracts and essential oil fraction was expressed as percent inhibition of DPPH radical (IC50). The results showed that the methanol extract was more active
(IC50 = 52.65) compared to n-hexane extract (IC50 = 470.17) while the essential oils
have the lowest antioxidant activity (IC50 = 479.48). Analysis of chemical components
in the essential oil of the leaves has been determined using Gas Chromatography-Mass
Spectroscopy (GC-MS) method. The main components of the essential oil are E-2-
octadecadecen-1-ol; methyl palmitate; n-hexadecanoic acid; methyl 11,14,17-
eicosatrienoate; and muurolol with the percentage yield according to the peak height
serially 26,66%, 16,35%, 8,66%, 8,13% and 5,94%
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Antioxidant activity, Annona squamosa, GC-MS