Biology

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Browsing Biology by Author "Aisyah, Nur"

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    OPTIMASI SUHU ANNEALING PASANGAN PRIMER UNTUK AMPLIFIKASI DAERAH ETS (EXTERNAL TRANSCRIBED SPACER) PADA TUMBUHAN DURIK-DURIK (Syzygium sp.) ASAL RIAU
    (2020-06) Aisyah, Nur; Roslim, Dewi Indriyani
    PCR (Polymerase chain reaction) is a technique for amplification target genes using specific primers. The PCR process consists of three stages, namely denaturation of template DNA, annealing and polymerization of DNA chain (extensions). The successful of a PCR is influenced by the PCR components and primer annealing temperature. This research was conducted to obtain optimal annealing temperature for the amplification of the ETS region. The PCR components were such as DreamTaq DNA Polymerase (Thermo Scientific) and a primer of Myrtf and 18SR. The optimal annealing temperature for PCR of the ETS region was 56,8°C. The amplicon obtained in this study was approximately 500 bp
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    PEMBUKTIAN DNA TOTAL TUMBUHAN TUNTUN ANGIN (Elaeocarpus floribundus) DENGAN SEKUEN GEN MATK
    (wahyu sari yeni, 2019-04-23) Aisyah, Nur; Herman, Herman
    Tuntun angin (Elaeocarpus floribundus) is one of important floodplain plant that grows in Kajuik Lake, Langgam, Pelalawan District, Riau Province, Indonesia. The ability of this plant for flooding stress adaption can’t be separated from the existence of genes related to the flooding stress. DNA isolation in this plant is useful in obtaining moleculer information that is useful for further analysis. The study aimed to prove that total DNA isolated was present by using matK gene. The isolation of total DNA was conducted by taking 0,1 g of tuntun angin leave. Methods included total DNA isolation, polymerase chain reaction (PCR), electrophoresis, sequencing and data analysis using bioinformatic tools use BioEdit versi 7.0 and BLASTn. Total DNA extraction used the Genomic DNA Mini Kit Plant (Geneaid). The total DNA was then migrated on 1 % agarose gel to know the quality of total DNA obtained. The total DNA of tuntun angin wasn’t detected on gel electrophoresis after visualizing on UV lamp. Amplification of matK gene was performed to proof that the total DNA was present. The result showed that a 529 bp of the matK gene was similar to Elaeocarpus floribundus available in GeneBank.