OPTIMASI SUHU ANNEALING PASANGAN PRIMER UNTUK AMPLIFIKASI DAERAH ETS (EXTERNAL TRANSCRIBED SPACER) PADA TUMBUHAN DURIK-DURIK (Syzygium sp.) ASAL RIAU
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Date
2020-06
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Abstract
PCR (Polymerase chain reaction) is a technique for amplification target genes using
specific primers. The PCR process consists of three stages, namely denaturation of template
DNA, annealing and polymerization of DNA chain (extensions). The successful of a PCR is
influenced by the PCR components and primer annealing temperature. This research was
conducted to obtain optimal annealing temperature for the amplification of the ETS region.
The PCR components were such as DreamTaq DNA Polymerase (Thermo Scientific) and a
primer of Myrtf and 18SR. The optimal annealing temperature for PCR of the ETS region
was 56,8°C. The amplicon obtained in this study was approximately 500 bp
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Keywords
annealing temperature, DNA polymerase, ETS region, PCR, Syzygium