PENGARUH PENAMBAHAN EDTA DAN SDS TERHADAP AKTIVITAS ENZIM LAKASE B Trichoderma asperellum LBKURCC1

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dc.contributor.authorWati, Laras
dc.contributor.authorDahliaty, Andi
dc.contributor.authorNugroho, Titania Tj
dc.date.accessioned2024-08-12T03:58:14Z
dc.date.available2024-08-12T03:58:14Z
dc.date.issued2023-12
dc.description.abstractLaccase enzyme is an extracellular enzyme that is catalyzing lignin degradation process. This study aims to analyze the effect of the addition of EDTA and SDS compounds on laccase enzyme activity with concentration variations of 1 mM and 10 mM. Laccase enzyme from Trichoderma asperellum LBKURCC1 had been partially purified by 0-80% ammonium sulfate fractionation and gel filtration chromatography where the results of this partial purification produced two kinds of laccase, namely laccase A and laccase B. This study used laccase B enzyme. The activity of laccase B enzyme was determined by UV-Vis spectrophotomoter at 420 nm wavelength using ABTS (2,2'-Azinobis-3-ethylbenzothiazolin-6- sulfonate) as substrate. The activity of laccase B enzyme was 144.02±2.71 U/L. Addition by 1 mM EDTA was not significantly different (p>0.05) with relative activity of (100.47±6.47)% control. A significant decrease in laccase activity (p≤0.05) occurred in the addition by EDTA 10 mM, SDS 1 mM and SDS 10 mM with relative activities of (76.58±2.6)%, (38.81±3.59)%, (8.98±1.35)% of the control, respectively.
dc.description.sponsorshipFakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau
dc.identifier.citationPerpustakaan
dc.identifier.otherElfitra
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/11403
dc.language.isoen
dc.publisherElfitra
dc.subjectActivity
dc.subjectEDTA
dc.subjectlaccase
dc.subjectSDS
dc.subjectTrichoderma asperellum
dc.titlePENGARUH PENAMBAHAN EDTA DAN SDS TERHADAP AKTIVITAS ENZIM LAKASE B Trichoderma asperellum LBKURCC1
dc.typeArticle

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