PENGARUH PENAMBAHAN EDTA DAN SDS TERHADAP AKTIVITAS ENZIM LAKASE B Trichoderma asperellum LBKURCC1
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Date
2023-12
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Publisher
Elfitra
Abstract
Laccase enzyme is an extracellular enzyme that is catalyzing lignin degradation
process. This study aims to analyze the effect of the addition of EDTA and SDS
compounds on laccase enzyme activity with concentration variations of 1 mM and
10 mM. Laccase enzyme from Trichoderma asperellum LBKURCC1 had been
partially purified by 0-80% ammonium sulfate fractionation and gel filtration
chromatography where the results of this partial purification produced two kinds
of laccase, namely laccase A and laccase B. This study used laccase B enzyme.
The activity of laccase B enzyme was determined by UV-Vis spectrophotomoter
at 420 nm wavelength using ABTS (2,2'-Azinobis-3-ethylbenzothiazolin-6-
sulfonate) as substrate. The activity of laccase B enzyme was 144.02±2.71 U/L.
Addition by 1 mM EDTA was not significantly different (p>0.05) with relative
activity of (100.47±6.47)% control. A significant decrease in laccase activity
(p≤0.05) occurred in the addition by EDTA 10 mM, SDS 1 mM and SDS 10 mM
with relative activities of (76.58±2.6)%, (38.81±3.59)%, (8.98±1.35)% of the
control, respectively.
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Keywords
Activity, EDTA, laccase, SDS, Trichoderma asperellum
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