SINTESIS DAN UJI TOKSISITAS SENYAWA (E)-7-(2- METOKSIBENZILIDEN)-3-(2-METOKSIFENIL)-2-FENIL-3, 3a, 4, 5, 6, 7- HEKSAHIDRO-2H-PIRAZOLO [4,3c] PIRIDIN

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2021-03

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Abstract

Pyrazoline is a dihydropyazole derivative which is included in the azole group of compounds with a heterocyclic-5 structure containing 2 nitrogen atoms, has various biological activities such as anticancer, antiinflation, antioxidants, and antimalarials. The purpose of this study was to synthesize the analog compound pyrazoline (E) -7- (2- methoxybenziliden) -3- (2-methoxyphenyl) -2-phenyl-3, 3a, 4, 5, 6, 7-hexahydro-2Hpyrazolo [4,3c] pyridine through the reaction of a pipiridon derivative curcumin analogue with phenylhydrazine using a sodium hydroxide catalyst. Curcumin compounds are obtained from a condensation reaction between 4-piperidone monohydrate hydrochloride and 2-methoxybenzaldehyde with a NaOH as catalyst. The pyrazoline were synthesized via sealed vessel reactor with a stirring speed of 1000 rpm 80oC yield of 53.15%. purity of the compound were analyzed using TLC, HPLC and melting point. Identification of the structure of pyrazoline compounds by UV, FTIR, NMR, and HRMS spectroscopy. The toxicity of pyrazoline compounds was tested by the BSLT method using A. salina. The test results showed that the pyrazoline compound is non-toxic with a value of LC50 = 674.06 μg / mL.

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pyrazoline, sealed vessel reactor, toxicity

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