OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH trnQ-5’rps16 INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAU

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dc.contributor.authorNurhayati, Siti
dc.contributor.supervisorRoslim, Dewi Indriyani
dc.date.accessioned2021-07-08T03:28:10Z
dc.date.available2021-07-08T03:28:10Z
dc.date.issued2020-06
dc.description.abstractPandan (Benstonea sp.) is one of the most important plant in Kajuik Lake located in Langgam, Riau Province, Indonesia. Polymerase Chain Reaction (PCR) is a technique used to multiply or amplify DNA samples in vitro using a primer pair. This study aims to optimize annealing temperature of trnQ-5’rps16 intergenic spacer primer. Stages of research are total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), PCR and electrophoresis. The result showed that the optimal annealing temperature for amplification of trnQ-5’rps16 intergenic spacer is 58°C. The PCR product on this annealing temperature is thick.en_US
dc.description.sponsorshipJurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/10005
dc.language.isoenen_US
dc.subjectAnnealing temperatureen_US
dc.subjectBenstonea spen_US
dc.subjectPCRen_US
dc.subjectRiauen_US
dc.subjecttrnQ-5’rps16 intergenic spaceren_US
dc.titleOPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH trnQ-5’rps16 INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAUen_US
dc.typeArticleen_US

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