OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH trnQ-5’rps16 INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAU
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Date
2020-06
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Abstract
Pandan (Benstonea sp.) is one of the most important plant in Kajuik Lake located
in Langgam, Riau Province, Indonesia. Polymerase Chain Reaction (PCR) is a
technique used to multiply or amplify DNA samples in vitro using a primer pair.
This study aims to optimize annealing temperature of trnQ-5’rps16 intergenic
spacer primer. Stages of research are total DNA isolation using Genomic DNA
Mini Kit Plant (Geneaid), PCR and electrophoresis. The result showed that the
optimal annealing temperature for amplification of trnQ-5’rps16 intergenic
spacer is 58°C. The PCR product on this annealing temperature is thick.
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Keywords
Annealing temperature, Benstonea sp, PCR, Riau, trnQ-5’rps16 intergenic spacer