Morphological Characteristic and Isolation of Germ Cell : A Preliminary Study for The Germ Cell Transplantation of Celebes Rainbow (Marosatherina ladigesi)

Simple item page
dc.contributor.authorAndriani, Irma
dc.date.accessioned2016-03-31T04:05:48Z
dc.date.available2016-03-31T04:05:48Z
dc.date.issued2016-03-31
dc.description.abstractThe recent study were attempting to develop germ cell transplantation as a tool to preserve and propagate male germplasm from endangered fish species, as well as to produce surrogatebroodstock of commercially valuable fish. Germ cell identification and gonada dissociation were the first necessary steps in germ cell transplantation. Female and male germ cells werecharacterized histologically. For germ cell dissociation, freshly isolated gonad of adult wereminced gently in medium with different enzyme. Medium A contained 0.5% trypsin andmedium B contained 0.1% colagenase then incubated for 2 hours to get monodisperce cellsuspension. Parameters observed were the diameter of both female and male germ cells of Celebes rainbow the abundance of spermatogonia and oogonia, the viability of spermatogoniaand oogonia after dissociation. The viability was analyzed using trypan blue exclusion dye. Histological analysis showed that testes and ovarium of adult Celebes rainbow (±5 cm) examinedwere still containing germ cell at all the stages of development. Spermatogonia mostly located close to the basement membrane of the germinal epithelium. The average number of spermatogonia observed in medium B was higher than in medium A (P<0.05), meanwhile theviability of spermatogonia between medium A and B were not significantly different (P>0.05).Key words : morphological characteristis, dissociation, viability, Celebes rainbow, transplantation.en_US
dc.description.sponsorshipInternational Seminar of Fisheries and Marine (2ndISFM 2013)en_US
dc.identifier.isbn978-979-792-437-9
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttp://repository.unri.ac.id/xmlui/handle/123456789/8141
dc.language.isoenen_US
dc.subjectgerm cellen_US
dc.subjectCelebes rainbowen_US
dc.subjecttransplantationen_US
dc.titleMorphological Characteristic and Isolation of Germ Cell : A Preliminary Study for The Germ Cell Transplantation of Celebes Rainbow (Marosatherina ladigesi)en_US
dc.typeUR-Proceedingsen_US

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
23 Full_paper_2ndISFM_Irma Andriani_UNHAS.pdf
Size:
185.71 KB
Format:
Adobe Portable Document Format
Description:
artikel
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

4. International Seminar of Fisheries and Marine (2ndISFM 2013)