Morphological Characteristic and Isolation of Germ Cell : A Preliminary Study for The Germ Cell Transplantation of Celebes Rainbow (Marosatherina ladigesi)
No Thumbnail Available
Date
2016-03-31
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The recent study were attempting to develop germ cell transplantation as a tool to preserve and propagate male germplasm
from endangered fish species, as well as to produce surrogatebroodstock of commercially valuable fish. Germ cell
identification and gonada dissociation were the first necessary steps in germ cell transplantation. Female and male germ
cells werecharacterized histologically. For germ cell dissociation, freshly isolated gonad of adult wereminced gently in
medium with different enzyme. Medium A contained 0.5% trypsin andmedium B contained 0.1% colagenase then
incubated for 2 hours to get monodisperce cellsuspension. Parameters observed were the diameter of both female and
male germ cells of Celebes rainbow the abundance of spermatogonia and oogonia, the viability of spermatogoniaand
oogonia after dissociation. The viability was analyzed using trypan blue exclusion dye. Histological analysis showed that
testes and ovarium of adult Celebes rainbow (±5 cm) examinedwere still containing germ cell at all the stages of
development. Spermatogonia mostly located close to the basement membrane of the germinal epithelium. The average
number of spermatogonia observed in medium B was higher than in medium A (P<0.05), meanwhile theviability of
spermatogonia between medium A and B were not significantly different (P>0.05).Key words : morphological characteristis,
dissociation, viability, Celebes rainbow, transplantation.
Description
Keywords
germ cell, Celebes rainbow, transplantation