Abstract:
Lipolytic bacteria are bacteria that produce lipase enzymes to hydrolyze fats into fatty acids
and glycerol. Lipolytic bacteria can be found in oil-contaminated habitats such as palm oil
mill effluent. One of the applications of lipase enzymes is as biodetergent formulations that
are eco-friendly. This research aimed to obtain potential lipolytic bacteria isolates from
palm oil mill effluent and analyze the potential of their lipase to be applied as a
biodetergent formulation through several tests, namely enzyme activity test, enzyme
activity stability test and washing test. The results obtained 22 isolates of lipolytic bacteria
with one isolate of potential lipolytic bacteria. LSSB 4.1 isolate produced lipase activity of
4.125 U/ml, produced a relative activity of 88% when the isolate was exposed to 1%
detergent and could remove oil from cotton fabric with a percentage of 61.3%. Lipase from
LSSB 4.1 isolates has the potential to be developed as a biodetergents formulation.
