ANALISIS TIGA METODE PENENTUAN AKTIVITAS ENZIM α-AMILASE
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Date
2021-03
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Abstract
The enzyme activity as a biocatalyst can be calculated based on the number of moles of
the substrate that is hydrolyzed into product per unit time (Iodine method), or the
number of moles of product produced per unit time (2,3-dinitrosalicylic acid (DNS)
method and the Nelson-Somogyi (NS) method). This third method are with
experimental conditions of pH 7, temperature of 50°C and an incubation time of 30
minutes. The results showed that the α-amylase enzyme activity measured by the DNS
method was 0.115 ± 0.002 μmol/minute and the NS method was 0.041 ± 0.025
μmol/minute, while the Iodine method had activity of 2.090 ± 0.005 μmol/minute. The
activity data of these three methods after being statistically tested by the Duncan
method turned out to be significant between the DNS method (2,3-dinitrosalicylic acid)
with the Nelson-Somogyi (NS) method and the Iodine method.
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Keywords
3,5-dinitrosalicylic acid, α-amylase enzyme, iodine, Nelson-Somogyi, strach