Abstract:
DNA barcoding techniques can be utilized in the field of plant taxonomy
and phylogenetics to identify plants more accurately. Polymerase Chain Reaction
(PCR) is a technology that supports to multiply a small sample of DNA into
millions of copies. Annealing temperature optimization needs to be done in order
to obtain specific target DNA. This research was conducted to obtain annealing
temperature to amplify the ndhF-rpl32 Intergenic Spacer region in pandanus
plants (Benstonea sp.) from Riau. The stages of this research included total DNA
isolation using Genomic DNA Mini Kit Plant, PCR replication using the
DreamTaq DNA Polymerase kit, and electrophoresis. The primers used in this
study were ndhF: 5'- GAA AGG TAT KAT CCA YGM ATA TT-3 ' and rpl32-R:
5'- CCA ATA TCC CTT YYT TTT CCA A-3 '. The results obtained from this
study showed that optimal annealing temperatures were found at 50,3
◦
C.
