6.International Seminar on Fisheries and Marine Science 2015

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Browsing 6.International Seminar on Fisheries and Marine Science 2015 by Subject "Hemibagrus nemurus"

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    Molecular Cloning and Phylogenetic Analysis of Vitellogenin Gene in Hemibagrus nemurus
    (2016-08-28) Ling, Ang Pay; Harmin, S.A.; Salwany, M.Y. Ina; O. Roshani; Z. Zulperi; S.N. Baharum; Syahril, M.Z. Mohd
    Vitellogenin is an egg yolk precursor glycophospolipoprotein, which it can be used as a biomarker indicator in sexual maturation. In this study, total of the nucleotide size for this partial VTG sequence is 574bp. The partial VTG nucleotide homology of Hemibagrus nemurus is high; showing 88-91% identity between Clarias macrocephalus and Ariopsis felis. The VTG sequences analysis has shown the highest homologous portion of the VTG gene between local H. nemurus with Ariopsis felis and Bagre marinus. VTG from different species are conserved domains, and phylogenetically related. Studies as such will certainly provide new insights to better understand the biological role of VTG in river catfish.
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    Phylogenetic Analysis of Vitellogenin Peptides in Hemibagrus nemurus
    (2016-08-28) Roshani O; Harmin S. A.; Salwany M. Y., Ina
    Vitellogenin is a female-specific glycolipophosphoproteins and it plays a significant role in embryo development and reproduction of Hemibagrus nemurus. The vitellogenin have been characterized to have various molecular weights in different fish species and has led to questions over ways to identify the vitellogenin peptides in river catfish that has been evolutionarily conserved in teleost. In this study, the amino acid sequences for the vitellogenin peptides for H. nemurus are AYLAGAAADVLEVGVR and AYLAGAAADVLDIGVR and were used to investigate the phylogenetic relationships between vitellogenin in different fish species in the database. The phylogenetic analysis based upon the vitellogenin peptides of H. nemurus indicates a close phylogenetic relationship with vitellogenin in Clarias batrachus. Vtg is a highly conserved protein and the molecular weight or size is species-specific, thus the similarity value was obtained due to the fact that both species belong to the Asian freshwater catfish family.