Phylogenetic Analysis of Vitellogenin Peptides in Hemibagrus nemurus

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2016-08-28

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Abstract

Vitellogenin is a female-specific glycolipophosphoproteins and it plays a significant role in embryo development and reproduction of Hemibagrus nemurus. The vitellogenin have been characterized to have various molecular weights in different fish species and has led to questions over ways to identify the vitellogenin peptides in river catfish that has been evolutionarily conserved in teleost. In this study, the amino acid sequences for the vitellogenin peptides for H. nemurus are AYLAGAAADVLEVGVR and AYLAGAAADVLDIGVR and were used to investigate the phylogenetic relationships between vitellogenin in different fish species in the database. The phylogenetic analysis based upon the vitellogenin peptides of H. nemurus indicates a close phylogenetic relationship with vitellogenin in Clarias batrachus. Vtg is a highly conserved protein and the molecular weight or size is species-specific, thus the similarity value was obtained due to the fact that both species belong to the Asian freshwater catfish family.

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vitellogenin, Hemibagrus nemurus, phylogenetic

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