Browsing by Author "Teruna, H. Y."

Now showing 1 - 6 of 6
  • No Thumbnail Available
    Item
    IDENTIFIKASI DAN UJI AKTIVITAS ANTIOKSIDAN FRAKSI DARI EKSTRAK N-HEKSANA DAUN TUMBUHAN Goniothalamus sesquipedalis
    (2013-06-28) Syainanda, V. K.; Teruna, H. Y.; Jose, C.
    In Indonesia, Goniothalamus sesquipedalis could be found in Hutan Lindung Bukit Suligi, Rokan Hulu Regency, Riau Province where the leaves of G. sesquipedalis were collected. An antioxidant activity determination of G. sesquipedalis leaves extracts had been done by using DPPH (1,1-diphenyl–2–picryl hydrazyl) method. The results showed that methanol extract was more active as antioxidant (IC50 = 88,0722 μg/mL ) compared to n-hexane extract (IC50 = 754,7705 μg/mL). The extract was obtained by 3 times macerating dried leaves powder of G. sesquipedalis by using n-hexane and methanol respectively. The n-hexane extract was fractionated by vacuum liquid chromatography (VLC) and the second fraction (F2) of VLC was continued to be fractionated by using Chromatotron. Fractions of F4-F8 of Chromatotron were combined to be one fraction because of same number of retardation factor (Rf) on thin layer chromatography (TLC) determination. Analysis and identification of chemical components in combined Chromatotron fraction of the n-hexane leaves extract were determined by using high performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC-MS). The main components of this fraction were α-humulene; (-)-sinularene; germacrene-D; δ-cadinene; α-selinene and neophytadiene. DDPH method was also used to determine antioxidant activity of this fraction. The result showed that combined Chromatotron fraction had the lowest antioxidant activity with %inhibition = 17,0421% (1000 μg/mL).
  • No Thumbnail Available
    Item
    IDENTIFIKASI DAN UJI AKTIVITAS TOKSISITAS EKSTRAK n-HEKSANA DARI KULIT BATANG TUMBUHAN Xylopia malayana Hook. f.et Thomson (ANNONACEAE)
    (2014-03-27) Amenda, A.; Teruna, H. Y.; Yuharmen
    The plant family of Annonaceae spreads out widely in Indonesia. This family is best known for its secondary metabolite resource. Xylopia malayana, a species of Annonaceae can be found in Pekanbaru. The objective of this study was to isolate secondary metabolite from the stem bark. It has been carried out using maceration method with n-hexane as the solvent. Separation of the metabolites has been done using vacuum liquid chromatography (VLC) and column chromatography. Nine combined fractions were produced The extract subjected to VLC were obtained nine combined by VLC fraction 1 (Fg 1) to fraction 9 (Fg 9). Fg 2 was analyzed using gas chromatography-mass spectrometry (GC-MS) resulting 35 peaks of coumpounds. This fraction contained some important volatile oils such as caryophyllene oxide (7,40%), 3,4-dimethyl, 3-cyclohexen- -carboxaldehyde (4,98%), valerenal (15,49%), 2-methyl-4- (2,6,6-trimethyl-1-cyclohexen-1-yl)-2-butenal (8,37%), hexadecanoic acid (4,31%) and oleic acid (8,14%). The result from toxicity test by BLST method of nine fractions showed only Fg 1 had high toxicity (LC50 = 50,11 ppm)
  • No Thumbnail Available
    Item
    ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL DARI DAUN TANAMAN Annona squamosa L
    (2013-03-09) Amalia, R.; Teruna, H. Y.; Balatif, N.
    An investigation on the antioxidant activity of Annona squamosa extract and essential oil has been done using DPPH ( 1,1- diphenyl – 2 – picryl hydrazyl) method. The extract was obtained by macerating the leaves of Annona squamosa using n-hexane and methanol respectively. The methanol extract was fractionated by vacuum liquid chromatography, resulted essential oil fractionation (Fraction 4). The antioxidant activity of the extracts and essential oil fraction was expressed as percent inhibition of DPPH radical (IC50). The results showed that the methanol extract was more active (IC50 = 52.65) compared to n-hexane extract (IC50 = 470.17) while the essential oils have the lowest antioxidant activity (IC50 = 479.48). Analysis of chemical components in the essential oil of the leaves has been determined using Gas Chromatography-Mass Spectroscopy (GC-MS) method. The main components of the essential oil are E-2- octadecadecen-1-ol; methyl palmitate; n-hexadecanoic acid; methyl 11,14,17- eicosatrienoate; and muurolol with the percentage yield according to the peak height serially 26,66%, 16,35%, 8,66%, 8,13% and 5,94%
  • No Thumbnail Available
    Item
    ISOLASI METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK METANOL DAUN TANAMAN SRIKAYA (Annona squamosa Linn)
    (2013-04-17) Tripiana, M.; Teruna, H. Y.; Balatif, N.
    An investigation on the toxicity of srikaya (Annona squamosa ) extract has been done by using the Brine Shrimp Lethality Test (BSLT). The extract was obtained by macerating the leaves of A. squamosa using n-hexane and methanol respectively. The methanol extract was fractionated by vacuum liquid chromatography method (VLC). The results showed that the extracts of n-hexane and methanol more toxic than the fraction 4.
  • No Thumbnail Available
    Item
    ISOLASI, UJI AKTIVITAS, DAN AKTIVITAS SPESIFIK ENZIM SELULASE Penicillium sp. LBKURCC27 SEMIMURNI MELALUI PENGENDAPAN (NH 4 ) 2SO 4
    (2014-03-27) Sarip, M.; Nugroho, T. T; Teruna, H. Y.
    Enzyme activity of cellulase can be concentrated by ammonium sulfate ((NH 4)2SO4) precipitation. The concentration was carried out by adding ((NH4)2SO4) 80% saturated precipitation. The precipitated enzyme was redisolved in 1/70 of the original crude extract volume. Enzyme activity of cellulase was assayed using CMC and avicel as the substrate. The results showed that in the concentrated enzyme preparation, the CMCase was increased 12 fold and enzyme activity of avicelase increased 3 fold. Activity and specific activity of the final CMCase preparation were 1.1160±0,0622 U/mL and 7.43±0,417 U/mg, respectively. Activity and specific activity of avicelase preparation were 0.0520±0.0735 U/mL and 0.34±0.487 U/mg, respectively.
  • No Thumbnail Available
    Item
    SINTESIS (E)-3-(4-FLUOROFENIL)-1-(NAFTALEN-1-IL)PROP-2-EN-1-ON DARI 1-ASETILNAFTALEN DAN 4-FLUOROBENZALDEHID
    (2014-03-27) Fitria, D.; Teruna, H. Y.; Jasril
    Chalcone analogues has been synthesized by aldol condensation using stirring method with a base catalyst (KOH). Starting material that has been used was 1- acetylnaphthalene as ketone and 4-fluorobenzaldehyde as aldehyde. This compound were (E)-3-(4-flurophenyl)-1-(naphthalen-1-yl)prop-2-en-1-one. The purity of the product has been evaluated by TLC, melting point test, and HPLC analysis. The structures of the synthesized compound were confirmed by UV, FTIR, NMR, and MS analysis. Data of characterization showed that the compound obtained was based on target compound