IDENTIFIKASI DAN UJI AKTIVITAS ANTIOKSIDAN FRAKSI DARI EKSTRAK N-HEKSANA DAUN TUMBUHAN Goniothalamus sesquipedalis
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Date
2013-06-28
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Abstract
In Indonesia, Goniothalamus sesquipedalis could be found in Hutan Lindung Bukit
Suligi, Rokan Hulu Regency, Riau Province where the leaves of G. sesquipedalis were
collected. An antioxidant activity determination of G. sesquipedalis leaves extracts had been done by using DPPH (1,1-diphenyl–2–picryl hydrazyl) method. The results
showed that methanol extract was more active as antioxidant (IC50 = 88,0722 μg/mL )
compared to n-hexane extract (IC50 = 754,7705 μg/mL). The extract was obtained by 3 times macerating dried leaves powder of G. sesquipedalis by using n-hexane and
methanol respectively. The n-hexane extract was fractionated by vacuum liquid
chromatography (VLC) and the second fraction (F2) of VLC was continued to be
fractionated by using Chromatotron. Fractions of F4-F8 of Chromatotron were
combined to be one fraction because of same number of retardation factor (Rf) on thin
layer chromatography (TLC) determination. Analysis and identification of chemical
components in combined Chromatotron fraction of the n-hexane leaves extract were
determined by using high performance liquid chromatography (HPLC) and gas
chromatography-mass spectroscopy (GC-MS). The main components of this fraction
were α-humulene; (-)-sinularene; germacrene-D; δ-cadinene; α-selinene and
neophytadiene. DDPH method was also used to determine antioxidant activity of this
fraction. The result showed that combined Chromatotron fraction had the lowest
antioxidant activity with %inhibition = 17,0421% (1000 μg/mL).
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Keywords
Goniothalamus sesquipedalis, GC-MS, Antioxidant activity