OPTIMALISASI pH PRODUKSI ENZIM AMILASE BAKTERI ENDOFITIK Pseudomonas stutzery LBKURCC46
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2016-10-19
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Abstract
Amylase is an hydrolase enzyme which hydrolyze starch into maltose and glucose. Amylase has been produced by Pseudomanas stutzery LBKURCC45, LBKURCC46, and LBKURCC55, the endophytic bacteria. Amylase activity was determined based on the reducing sugars formed from starch hydrolisis by amylase with the DNS (dinitrosalicylic acid) method on a UV-Vis spectrophotometer at 540 nm. The highest amylase activity at pH 7.0, 40°C and agitation of 120 rpm was shown by LBKURCC46 bacteria. Furthermore, the optimization of amylase production by LBKURCC46 bacteria was conducted at various pHs (6.0; 6.5; 7.0; 7.5). The results showed that the optimum activity was 27.5308 x 10-3 U/mL at pH 6.5.
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Amylase, DNS, Pseudomanas stutzery