OPTIMASI SUHU ANNEALING PASANGAN PRIMER UNTUK AMPLIFIKASI DAERAH atpB-rbcL INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAU

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dc.contributor.authorNuraini, Intan Sari
dc.contributor.supervisorRoslim, Dewi Indriyani
dc.date.accessioned2021-07-08T03:13:55Z
dc.date.available2021-07-08T03:13:55Z
dc.date.issued2020-06
dc.description.abstractAnnealing temperature is a temperature that is required for primer annealing in DNA template. This temperature is very crucial for the PCR success. This study aims to determine the appropriate annealing temperature for amplifying of atpB-rbcL intergenic spacer on Benstonea sp. The PCR components use Dream Taq DNA Polymerase (Thermo Scientific) and a primer pair of atpB_F and rbcL_R. The optimal annealing temperature for PCR of the atpB-rbcL intergenic spacer was 50,2 °C. The amplificon on this annealing temperature was single, thick and the size is right.en_US
dc.description.sponsorshipBiologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/9999
dc.language.isoenen_US
dc.subjectannealing temperatureen_US
dc.subjectatpB-rbcL intergenic spaceren_US
dc.subjectBenstonea spen_US
dc.subjectPCRen_US
dc.titleOPTIMASI SUHU ANNEALING PASANGAN PRIMER UNTUK AMPLIFIKASI DAERAH atpB-rbcL INTERGENIC SPACER PADA TUMBUHAN PANDAN (Benstonea sp.) ASAL RIAUen_US
dc.typeArticleen_US

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