SINTESIS DAN UJI AKTIVITAS ANTIDIABETES SENYAWA ETIL-2- (3-(3-BROMOFENIL)-6-OKSOPIRIDAZIN-1(6H)-IL)ASETAT SECARA IN SILICO DAN IN VITRO

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dc.contributor.authorOktadini, Dewi
dc.contributor.supervisorZamri, Adel
dc.date.accessioned2022-01-17T03:24:14Z
dc.date.available2022-01-17T03:24:14Z
dc.date.issued2021-07
dc.description.abstractDiabetes mellitus is a chronic disease with impaired carbohydrate, fat and protein metabolism caused by decreased insulin sensitivity and secretion. Pyridazinone is a dinitrogen aromatic ring compound with one carbonyl which has various biological activities, one of which is antidiabetic. This research aims to synthesize pyridazinone derivatives and determine the antidiabetic activity through approaches in silico (molecular docking) and in vitro. The compound ethyl-2-(3-(3-bromophenyl)-6- oxopyridazine-1(6H)-yl)acetate was successfully synthesized using the method stirrer at room temperature from a substitution reaction between the compound 6-(3- bromophenyl)pyridazine-3(2H)-one with ethyl chloroacetate. The purity of the compound was determined by TLC test, melting point measurement and HPLC. The structure of the synthesized compound was confirmed by spectroscopic analysis of UV, FTIR, 1H-NMR and HRMS. The synthesized compounds were tested for antidiabetic activity in silico (molecular docking) and in vitro. Study was molecular docking carried out on the crystal structure of human lysosomal acid-α-glucosidase in complex with moranolin from the database (PDB-ID 5NN5) and in vitro of the enzyme α-glucosidase. The activity of the ethyl-2-(3-(3-bromophenyl)-6-oxopyridazine-1(6H)-yl)acetate was not active as an inhibitor of the α-glucosidase enzyme with IC50 > 2000 μg/mL and confirmed through this study in silico (molecular docking) with binding free energy -11.457 kcal/mol, and only had two hydrogen bond interactions in common with positive control of acarbose, amino acid residues His674 and Arg672.en_US
dc.description.sponsorshipDosen Bidang Kimia Organik Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/10386
dc.language.isoenen_US
dc.publisherperpustakaan URen_US
dc.subjectantidiabeticen_US
dc.subjectin silicoen_US
dc.subjectin vitroen_US
dc.subjectpyridazinoneen_US
dc.titleSINTESIS DAN UJI AKTIVITAS ANTIDIABETES SENYAWA ETIL-2- (3-(3-BROMOFENIL)-6-OKSOPIRIDAZIN-1(6H)-IL)ASETAT SECARA IN SILICO DAN IN VITROen_US
dc.typeArticleen_US

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