TRANSFORMATION OF GENE TcPIN THE EXPRESSION VECTOR pBIN+ FOR SOYBEAN POD BORER RESISTANCE
dc.contributor.author | Isda, Mayta Novaliza | |
dc.contributor.author | Chaidamsari, Tetty | |
dc.contributor.author | Lestari, Wahyu | |
dc.date.accessioned | 2014-05-22T03:20:33Z | |
dc.date.available | 2014-05-22T03:20:33Z | |
dc.date.issued | 2014-05-22 | |
dc.description.abstract | Potential of gene resistance found in cocoa like proteinase inhibitor (pin) has an important role in plant defense system against predators and pathogens. Utilization of the pin is a new alternative to soybean in pod borer resistance. Proteinase Inhibitor (PIN) is known as a part of the plants defences system towards the pest. The research is conduct to construct TcPIN gene from cocoa crop into expression pBIN+ vector. The first step of the research is designed a new primary for the TcPIN gene construction. The TcPIN gene yielded from the purification of PCR product is construct into a flap plasmid completed with promotor and terminator. And as the next step, the gene fragment is constructed into pBIN+ and transformed inside competence cell of XL-1 Blue to multiply the plasmid. The result of the amplification is digested with the restrict enzyme SalI and BamHI to exposed the insert (PIN gene) from pGEMT-easy vector. The exposed insert (PIN gene) is transform by pFlap before transferred into the expression vector which is pBIN+. The result showed that the gene TcPIN has been inserted into pBIN+. The construction of the recombinant DNA shown with the digestion result of pBIN+, after cutting with Asc1 and Pac1 restrict enzyme | en_US |
dc.description.sponsorship | Seminar UR-UKM ke-7 2012 | en_US |
dc.identifier.isbn | 978-602-18936-0-9 | |
dc.identifier.other | wahyu sari yeni | |
dc.identifier.uri | http://repository.unri.ac.id/xmlui/handle/123456789/6305 | |
dc.language.iso | en | en_US |
dc.subject | TcPIN gene | en_US |
dc.subject | soybean pod borer | en_US |
dc.subject | gene constuction | en_US |
dc.subject | proteinase inhibitor | en_US |
dc.title | TRANSFORMATION OF GENE TcPIN THE EXPRESSION VECTOR pBIN+ FOR SOYBEAN POD BORER RESISTANCE | en_US |
dc.type | UR-Proceedings | en_US |
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