OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI GEN ndhF PADA TUMBUHAN DURIK-DURIK (Syzygium sp.)

Simple item page
dc.contributor.authorFitriani, Ana
dc.contributor.supervisorRoslim, Dewi Indriyani
dc.date.accessioned2021-07-15T03:19:03Z
dc.date.available2021-07-15T03:19:03Z
dc.date.issued2020-07
dc.description.abstractDurik-durik plant is categorized as a member of Syzygium genus based on DNA barcoding analysis using intergenic spacer psbA-trnH, matK, rbcl, ITS and trnL-F intergenic spacer. Polymerase Chain Reaction (PCR) is an in vitro DNA amplification technique using a thermal cycler machine to amplify DNA fragments in a relatively short time. PCR process is influenced by the PCR components and the annealing temperature. This research was conducted to optimize annealing temperature of 748F/1318R primer pair. Temperature optimization is analyzed in various annealing temperature. Result showed that the optimum temperature for amplification of ndhF gene is 46.6°C and the amplificon obtained was 600 bp.en_US
dc.description.sponsorshipJurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/10028
dc.language.isoenen_US
dc.subjectAmplificationen_US
dc.subjectdurik-duriken_US
dc.subjectSyzygium spen_US
dc.subjectPolymerase Chain Reactionen_US
dc.titleOPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI GEN ndhF PADA TUMBUHAN DURIK-DURIK (Syzygium sp.)en_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
REPOSITORY ANA FITRIANI_compressed.pdf
Size:
155.22 KB
Format:
Unknown data format
Description:
artikel
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Biology