SKRINING ANTIOKSIDAN EKSTRAK DAUN BENALU KOPI (Loranthus ferrugineus) DENGAN METODE DPPH
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Date
2021-09
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perpustakaan UR
Abstract
A coffee tree parasite is one of the Loranthaceae family which is distibuted in Indonesia.
This plant is widely used as a traditional medicine by various tribes. In previous studies
it was reported that several species of this genus have antimalarial, antioxidant,
antibacterial and anti-inflammatory properties. This study aims to determine the content
of secondary metabolites and determine antioxidants from coffee parasite extract.
Phytochemical tests were carried out on the coffee parasite plant to determine the content
of the secondary metabolite active compounds contained in the coffee parasite leaves.
Isolation of the compound was carried out by maceration method using ethyl acetate as
solvent. The antioxidant activity test was carried out using the 1,1-diphenyl-2-
picrylhydrazyl (DPPH) method. Based on the results of phytochemical tests, the
metabolites contained are phenolic, saponins, steroids, and terpenoids .Based on the
antioxidant activity tests, the IC50 value was higher than >1000 μg/mL (AAI= 0,000009),
ethyl acetate extract has very weak antioxidant activity IC50 348.19 g/mL (AAI = 0.23)
and methanol has weak antioxidant activity IC50 176,49 μg/mL (AAI= 0,45). Based on
the results of the research that has been done, it can be concluded that the parasite coffee
leaf plant has antioxidant activity.
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Keywords
antioxidant, coffee parasite, DPPH