POLA TIC (Total Ion Current) LCMS-MS EKSTRAK ETILASETAT DARI PRODUKSI METABOLIT SEKUNDER Penicillium sp. LBKURCC34 DENGAN VARIASI KONSENTRASI GLUKOSA
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2020-04
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Abstract
Liquid chromatography - mass spectrometry (LCMS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometryis. This tool could identify the differences of extract contains. The differences of secondary metabolites that are produced by co-culture of Penicillium sp. LBKURCC34 and Staphylococcus aureus with variation of glucose concentration (5g/L (Extract A), 10g/L (Extract B) and 15 g/L(Extract C)) were identified by LCMS. This study analyzed the difference patterns of Total Ion Current (TIC) of LCMS chromatogram profiles from these extracts. Based on this research, it was known that three extracts have different pattern of LCMS chromatogram with mostly of the peaks showed polar substances with molecular weight around 84,96 - 655,37 m/z.
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Penicillium sp, LBKURCC34, Co-culture extract, LCMS