AMPLIFIKASI GEN EF1α PADA TUMBUHAN TUNTUN ANGIN (Elaeocarpus floribundus)

Simple item page
dc.contributor.authorMutiarawati, Dilla
dc.contributor.authorHerman, Herman
dc.date.accessioned2019-04-23T02:09:45Z
dc.date.available2019-04-23T02:09:45Z
dc.date.issued2019-04-23
dc.description.abstractThe method of amplification is used in the genetic field, specifically to multiply target DNA, using PCR (polymerase chain reaction) method. To amplify the target DNA, the first step that must be done is DNA isolation. One of the target DNA that can be amplified is the EF1α gene in tuntun angin plant (Elaeocarpus floribundus), this gene is one of housekeeping gene. The amplification of the EF1α gene used a pair of primer EF1α_F : 5 '- TCC AGC TAA GGG TGC C -3' and EF1α_R : 5 '- GGT GGG TAC TCG GAG A -3', with annealing temperature of 54,70C. The PCR result was then checked using electrophoresis method. The results showed a band of the EF1α gene. The size of the EF1α gene band was determined using 1Kb ladder DNA. Size of EF1α gene band was found to be about 900 pb, with a thin and single band.en_US
dc.description.sponsorshipJURUSAN BIOLOGI FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM UNIVERSITAS RIAUen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/9655
dc.language.isoenen_US
dc.publisherwahyu sari yenien_US
dc.subjectAmplificationen_US
dc.subjectEF1α geneen_US
dc.subjectElaeocarpus floribundusen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjecttuntun anginen_US
dc.titleAMPLIFIKASI GEN EF1α PADA TUMBUHAN TUNTUN ANGIN (Elaeocarpus floribundus)en_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
DILLA MUTIARAWATI NIM. 1503114773.pdf
Size:
2.45 MB
Format:
Unknown data format
Description:
artikel
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Biology