PENENTUAN AKTIVITAS ENZIM LAKASE T. asperellum LBKURCC1 YANG TELAH DIFRAKSINASI SECARA PARSIAL MENGGUNAKAN AMONIUM SULFAT

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dc.contributor.authorAmalita, Lisna
dc.contributor.supervisorDahliaty, Andi
dc.date.accessioned2021-10-19T02:49:50Z
dc.date.available2021-10-19T02:49:50Z
dc.date.issued2021-03
dc.description.abstractLaccase are extracellular enzymes containing metal ions which used oxygen compounds to catalyze the oxidations process of various aromatic and nonaromatic compounds. The purpose of the study was to purify and determining laccase spesific activity from the isolate fungus T. asperellum LBKURCC1. Laccase enzymes purification process was carried out by teo steps, that was fractionation with ultrafiltraton membranes 10.000 MWCO (Molecular Weight Cut Of) and saturated with the addition of ammonium sulfate salts with saturation variations of 0-20%, 20- 40%, 40-60% and 60-80%. Based on this research, the results obtained were the activity of crude extract laccase of 0,01955 U/L, and highest enzymes activity was the enzyme purified by ammonium sulfat salt 0-20% saturation fraction of 0,03055 U/L. Specific activity of crude extract laccase enzyme was obtained at 4,4353 U/mg and the specific activity of purified enzymes decreased 2,1962 U/mg with a yield of 157,1296%. The results showed that enzymes in the saturation fraction of 0-20% ammonium sulfate was high activity and purity.en_US
dc.description.sponsorshipBidang Biokimia Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/10272
dc.language.isoenen_US
dc.subjectamonium sulfateen_US
dc.subjectspesific activityen_US
dc.subjectpurificationen_US
dc.titlePENENTUAN AKTIVITAS ENZIM LAKASE T. asperellum LBKURCC1 YANG TELAH DIFRAKSINASI SECARA PARSIAL MENGGUNAKAN AMONIUM SULFATen_US
dc.typeArticleen_US

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