POLA TIC (Total Ion Current) LC-MS/MS EKSTRAK ETIL ASETAT METABOLIT SEKUNDER DARI MEDIA PERTUMBUHAN Penicillium sp. LBKURCC34 DENGAN VARIASI WAKTU INKUBASI
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Date
2020-08
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Abstract
Liquid Chromatography Mass Spectrometry (LC-MS / MS) is an analytical technique
that combines the physical separation capabilities of liquid chromatography with the
specificity of mass spectrometry detection. This method can identify the differences of
compound from the extracts. The ethyl acetate extracts produced from the co-culture of
Penicillium sp. LBKURCC34 and Staphylococcus aureus with various incubation times
(8 days, 11 days and 14 days) were identified with LC-MS. The results of secondary
metabolites obtained showed that the peak of the dominant compound in extracts
generally was at retention times ranging from 9-13 min. The data showed that the
compound characteristics are more polar. Interestingly, only extract from 14 days
incubation contained less polarity compound.
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Keywords
incubation time, LC-MS analysis, secondary metabolites, Penicillium sp, LBKURCC34